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Rat Runt-related transcription factor 2(RUNX2) ELISA kit

  • 中文名称：
    大鼠Runt相关转录因子2(RUNX2)酶联免疫试剂盒
  • 货号：
    CSB-EL020594RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Runt-related transcription factor 2 (RUNX2) ELISA kit is a powerful tool for the quantitative measurement of RUNX2 protein levels in serum, plasma, tissue homogenates, and cell lysates of Rattus norvegicus (Rat) samples.

    RUNX2, also known as CBFA1, is a transcription factor that plays a critical role in bone development and remodeling，signal pathway. Our ELISA kit is specifically designed for the measurement of RUNX2 protein levels, making it an ideal tool for studying the molecular mechanisms underlying bone formation and skeletal disorders in rats.

    With a detection range of 3.12 ng/mL to 200 ng/mL and a sensitivity of 0.78 ng/mL, our RUNX2 ELISA kit offers exceptional accuracy and precision in measuring protein levels. The assay time is only 1-5 hours, and the sample volume required is just 50-100ul. The detection wavelength is 450 nm, and the assay principle is quantitative, utilizing a sandwich method for precise measurements.

    Our RUNX2 ELISA kit is suitable for researchers studying epigenetics and nuclear signaling in the context of bone development and disease. It is user-friendly and easy to use, providing reliable results for the research applications.

  • 别名：
    Runx2 ELISA Kit; Cbfa1 ELISA Kit; Osf2 ELISA Kit; Runt-related transcription factor 2 ELISA Kit; Core-binding factor subunit alpha-1 ELISA Kit; CBF-alpha-1 ELISA Kit; Osteoblast-specific transcription factor 2 ELISA Kit; OSF-2 ELISA Kit; Fragments ELISA Kit
  • 缩写：
    RUNX2
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat RUNX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 91-103  
    1:2 Average % 102  
    Range % 94-107  
    1:4 Average % 88  
    Range % 83-91  
    1:8 Average % 94  
    Range % 84-98  
  • 回收率：
    The recovery of rat RUNX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 103 93-107  
    EDTA plasma (n=4) 98 90-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    200 2.551 2.452 2.502 2.322  
    100 2.126 1.996 2.061 1.881  
    50 1.359 1.341 1.350 1.170  
    25 0.793 0.818 0.806 0.626  
    12.5 0.474 0.481 0.478 0.298  
    6.25 0.395 0.403 0.399 0.219  
    3.12 0.266 0.257 0.262 0.082  
    0 0.182 0.178 0.180    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
  • 亚细胞定位：
    Nucleus.
  • 数据库链接：