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  4. Rat Rheumatoid Factor(RF) ELISA Kit

Rat Rheumatoid Factor(RF) ELISA Kit

  • 中文名称：
    大鼠类风湿因子(RF)酶联免疫试剂盒
  • 货号：
    CSB-E13666r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Rheumatoid Factor (RF) ELISA Kit was designed for the quantitative measurement of Rat Rheumatoid Factor (RF) protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 mIU/mL-1000 mIU/mL and the sensitivity is 3.9 mIU/mL .

  • 别名：
    N/A
  • 缩写：
    RF
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    15.6 mIU/mL-1000 mIU/mL
  • 灵敏度：
    3.9 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat RF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %104
    Range %96-108
    1:10Average %94
    Range %91-98
    1:20Average %102
    Range %98-106
    1:40Average %88
    Range %82-96
  • 回收率：
    The recovery of rat RF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8983-95
    EDTA plasma (n=4)9992-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    10002.646 2.545 2.596 2.443
    5001.975 2.076 2.026 1.873
    2501.284 1.308 1.296 1.143
    1250.751 0.762 0.757 0.604
    62.50.512 0.501 0.507 0.354
    31.20.353 0.342 0.348 0.195
    15.60.285 0.281 0.283 0.130
    00.153 0.152 0.153 　
  • 数据处理：
  • 货期：
    3-5 working days

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