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  4. Rat Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

Rat Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

  • 中文名称：
    大鼠肺表面活性物质相关蛋白C(SP-C)酶联免疫试剂盒
  • 货号：
    CSB-E12631r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat SFTPC ELISA Kit was designed for the quantitative measurement of Rat SFTPC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.69 pg/mL-300 pg/mL and the sensitivity is 1.17 pg/mL.

  • 别名：
    Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit
  • 缩写：
    SFTPC
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    4.69 pg/mL-300 pg/mL
  • 灵敏度：
    1.17 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 85
    Range % 80-90
    1:2000 Average % 104
    Range % 100-109
    1:4000 Average % 91
    Range % 85-96
    1:8000 Average % 96
    Range % 91-103
  • 回收率：
    The recovery of rat SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 98 94-102
    EDTA plasma (n=4) 87 83-91
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    300 2.402 2.274 2.338 2.227
    150 1.939 2.051 1.995 1.884
    75 1.411 1.497 1.454 1.343
    37.5 0.939 0.904 0.922 0.811
    18.75 0.568 0.579 0.574 0.463
    9.38 0.355 0.382 0.369 0.258
    4.69 0.248 0.260 0.254 0.143
    0 0.114 0.108 0.111 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • 基因功能参考文献：
    1. SP-C is present on the surface of exocytosed surfactant particles. Antibodies against SP-C stabilize the released particles when contacting the air-liquid interface, precluding their transformation and the formation of surface films. SP-C acts as an essential, preformed molecular key in the initial stages of surfactant unpacking and surface film formation. PMID: 27155084
    2. misfolded surfactant protein C has a role in endoplasmic reticulum stress in epithelial-mesenchymal transition of alveolar epithelial cells PMID: 21169555
    3. Results suggest that there is no primary deficiency of surfactant proteins A, B, or C in the nitrofen-induced rat model of congenital diaphragmatic hernia. PMID: 12904592
    4. surfactant protein C processing requires a type II transmembrane topology directed by juxtamembrane positively charged residues PMID: 12933801
    5. NO mediated the decrease in surfactant protein gene expression PMID: 15640287
    6. Data indicate that heparin can be stimulatory to surfactant protein B and C gene expression depending on concentration, degree of sulfation, and surrounding fibroblast growth factor environment. PMID: 16794256

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  • 亚细胞定位：
    Secreted, extracellular space, surface film.
  • 数据库链接：

    KEGG: rno:50683

    STRING: 10116.ENSRNOP00000015032

    UniGene: Rn.3926