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  4. Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

  • 中文名称：
    大鼠肺表面活性物质相关蛋白B(SP-B)酶联免疫试剂盒
  • 货号：
    CSB-E12630r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit is a powerful research tool designed to detect and quantify Pulmonary surfactant-associated protein B in rat serum, plasma, and tissue homogenates. This kit is specifically designed for use in lung-related disease and signal transduction research and can detect levels of SP-B as low as 0.78 pg/mL with a detection range of 3.12 pg/mL to 200 pg/mL.

    The assay principle is based on the sandwich technique, in which the capture antibody is pre-coated on a microplate and the detection antibody is labeled with biotin. Upon binding to the target analyte, the complex is captured by streptavidin-HRP conjugate and the substrate TMB is added to produce a colored reaction product that is quantified by measuring the absorbance at 450 nm.

    With an assay time of 1-5 hours, a sample volume of 50-100 µl, and a detection wavelength of 450 nm, the SP-B ELISA kit provides accurate and reliable results for your research needs. With its high sensitivity and wide detection range, it can help you gain a better understanding of the role of SP-B in various physiological and pathological conditions.

  • 别名：
    Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
  • 缩写：
    SFTPB
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %90
    Range %85-100
    1:2000Average %94
    Range %87-98
    1:4000Average %90
    Range %85-96
    1:8000Average %94
    Range %90-98
  • 回收率：
    The recovery of rat SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-101
    EDTA plasma (n=4)9185-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.470 2.589 2.530 2.377
    1002.115 2.124 2.120 1.967
    501.713 1.726 1.720 1.567
    251.315 1.356 1.336 1.183
    12.50.985 0.971 0.978 0.825
    6.250.532 0.541 0.537 0.384
    3.120.378 0.389 0.384 0.231
    00.152 0.154 0.153 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
  • 基因功能参考文献：
    1. SP-B is present on the surface of exocytosed surfactant particles. Antibodies against SP-B stabilize the released particles when contacting the air-liquid interface, precluding their transformation and the formation of surface films. SP-B acts as an essential, preformed molecular key in the initial stages of surfactant unpacking and surface film formation. PMID: 27155084
    2. Liraglutide activates the GLP1 receptor, increasing ACE2 expression, reversing right ventricle hypertrophy, and improving the production of SP-A and SP-B in the lungs of Type 1 Diabetes rats PMID: 26196539
    3. The role of the SP-B NH(2) terminus in the formation and stability of cylindrical structures, specifically the distribution of lipid stack height, width, and density with four SP-B truncation peptides, was studied. PMID: 20023175
    4. VEGF increased expression of SP-b mRNA in preterm rat lung. PMID: 17267143
    5. alterations of surfactant are a consequence of the ventilation strategy that impair the biophysical activity of this material and thereby contribute directly to lung dysfunction over time PMID: 18344412

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  • 亚细胞定位：
    Secreted, extracellular space, surface film.
  • 数据库链接：