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  4. Rat Prostaglandin-H2 D-isomerase(PTGDS) ELISA kit

Rat Prostaglandin-H2 D-isomerase(PTGDS) ELISA kit

  • 中文名称：
    大鼠前列腺素-H2 D-异构酶(PTGDS)酶联免疫试剂盒
  • 货号：
    CSB-EL018969RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat PTGDS ELISA Kit was designed for the quantitative measurement of Rat PTGDS protein in serum, plasma, cerebrospinal fluid (CSF), tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.37 ng/mL-600 ng/mL and the sensitivity is 2.34 ng/mL.

  • 别名：
    Ptgds ELISA Kit; Prostaglandin-H2 D-isomerase ELISA Kit; EC 5.3.99.2 ELISA Kit; Glutathione-independent PGD synthase ELISA Kit; Lipocalin-type prostaglandin-D synthase ELISA Kit; Prostaglandin-D2 synthase ELISA Kit; PGD2 synthase ELISA Kit; PGDS ELISA Kit; PGDS2 ELISA Kit
  • 缩写：
    PTGDS
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
  • 检测范围：
    9.37 ng/mL-600 ng/mL
  • 灵敏度：
    2.34 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PTGDS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 90-100
    1:2 Average % 90
    Range % 85-94
    1:4 Average % 85
    Range % 80-89
    1:8 Average % 102
    Range % 98-107
  • 回收率：
    The recovery of rat PTGDS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 90-98
    EDTA plasma (n=4) 87 82-91
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    600 2.601 2.495 2.548 2.345
    300 2.004 1.916 1.960 1.757
    150 1.295 1.328 1.312 1.109
    75 0.817 0.860 0.839 0.636
    37.5 0.479 0.452 0.466 0.263
    18.75 0.352 0.339 0.346 0.143
    9.37 0.268 0.281 0.275 0.072
    0 0.199 0.207 0.203 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophobic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. Involved in PLA2G3-dependent maturation of mast cells. PLA2G3 is secreted by immature mast cells and acts on nearby fibroblasts upstream to PTDGS to synthesize PGD2, which in turn promotes mast cell maturation and degranulation via PTGDR.
  • 基因功能参考文献：
    1. HIF-1alpha-l-PGDS-PPARgamma regulates hypoxia-induced ANP secretion in beating rat atria PMID: 29287795
    2. Lipocalin-type prostaglandin synthase (L-PGDS) may have a role in sleep and chronic intermittent hypoxia PMID: 28025147
    3. Anorectic tumor-bearing rats do not display the changes in ventral striatal prostaglandin D synthase expression observed in food-restricted weight-matched animals. PMID: 21334429
    4. Gene expression is regulated by Hes-1 through E-box and interleukin-1 beta via two NF-kappa B elements in rat leptomeningeal cells. PMID: 12488457
    5. study showed that L-PGDS was strikingly expressed in the caput epididymidis, while a moderate to weak expression was observed in the corpus and cauda epididymidis PMID: 16730417
    6. Prostaglandin D synthase and prostacyclin synthase are present and could be regulated by steroids in the rat uterus during pregnancy. Endometrium could be significant source of PGD(2) and PGI(2) at specific times during pregnancy. PMID: 17951541
    7. These results suggested that the removal of the disulfide bond slightly increased the affinities of L-PGDS for ligand binding by changing the hydrophobic regions. PMID: 18590794
    8. This cardioprotective effect of dexamethasone was markedly reduced in L-PGDS-deficient mice PMID: 19451694

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  • 亚细胞定位：
    Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm, perinuclear region. Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. In the eye, it is expressed in the pigmented epithelium of the retina and the nonpigmented epithelium of the iris and ci
  • 数据库链接：