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  4. Rat Parathyroid hormone,PTH ELISA Kit

Rat Parathyroid hormone,PTH ELISA Kit

  • 中文名称：
    大鼠甲状旁腺激素(PTH)酶联免疫试剂盒
  • 货号：
    CSB-E07866r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat PTH ELISA Kit was designed for the quantitative measurement of Rat PTH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

  • 别名：
    Pth ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %97
    Range %91-103
    1:10Average %99
    Range %95-107
    1:20Average %95
    Range %90-99
    1:40Average %87
    Range %81-92
  • 回收率：
    The recovery of rat PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-97
    EDTA plasma (n=4)106102-109
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.613 2.511 2.562 2.408
    2002.054 1.953 2.004 1.850
    1001.276 1.287 1.282 1.128
    500.727 0.738 0.733 0.579
    250.457 0.468 0.463 0.309
    12.50.361 0.350 0.356 0.202
    6.250.275 0.269 0.272 0.118
    00.154 0.153 0.154 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

大鼠PTH ELISA试剂盒是否可拆卸？

A:
是，酶标板是可拆卸的

靶点详情

  • 功能：
    PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • 基因功能参考文献：
    1. PTHrP and PTH mediate wasting through a common mechanism involving PTHR. PMID: 26669699
    2. our data demonstrate that PTH activation of nuclear receptor-associated protein 1 is essential for the effect of PTH to increase FGF23 levels. PMID: 24940803
    3. data support a link between PTH regulating HDAC4 phosphorylation by PKA, trafficking, partial degradation, and the control of MMP-13 transcription through association with Runx2 PMID: 24904057
    4. Mg reduces PTH secretion mainly when a moderate low calcium concentration is present; Mg also modulates parathyroid glands function through upregulation of the key cellular receptors CaR, VDR and FGF23/Klotho system. PMID: 24103811
    5. The persistence of the phosphaturic response to PTH occurs through posttranscriptional mechanisms. PMID: 23344572
    6. The secretory response of parathyroid hormone to acute hypocalcemia in vivo is independent of parathyroid glandular sodium/potassium-ATPase activity. PMID: 21209610
    7. Data suggest that a high concentration of PTH(1-34)-induced cytotoxicity and apoptosis in PC12 cells was associated with upregulation of ERK and p38 through a mitochondria-mediated apoptotic pathway. PMID: 20971569
    8. Study indicates an influence of sex on PTH molecular form secretion or metabolism. PMID: 20627105
    9. Modulation of integrin expression in osteoblastic cells by PTH is likely to be indirect, and phenotypic expression of integrin subtypes is part of the cascade of events that lead to PTH (1-34) mediated osteoblast differentiation. PMID: 11746505
    10. New signaling pathway for parathyroid hormone and cyclic AMP action on extracellular-regulated kinase and cell proliferation in bone cells PMID: 11956184
    11. alterations in PI3K activity in response to parathyroid hormone with aging PMID: 12581861
    12. PTH or PTHrP can exert anabolic effects in the skeleton. PMID: 12631114
    13. that periosteal and endosteal osteoblastic cell populations exhibit metabolic differences in their extracellular matrix responses to PTH PMID: 14514685
    14. the parathyroid hormone-Smad3 axis may have a role in the bone anabolic action of PTH PMID: 14517210
    15. PTH and mechanical stimulation on trabecular bone formation rate suggest a potential benefit for combined PTH administration and exercise in the treatment of osteoporosis. PMID: 14672346
    16. Rho and Rho kinase are an important component of PTH signaling in osteoblastic cells PMID: 15476589
    17. Combinations of radioprotectors may achieve their beneficial additive effects in the growth plate by decreasing the usual early drop in parathyroid hormone-related protein and parathyroid hormone receptor 1 after irradiation PMID: 16494524
    18. Calcium, phosphate & uremia determine parathyroid hormone mRNA stability through binding of protective factors adenosine-uridine rich binding factor 1 & Up-stream of N-ras & recruitment of degradation complex that cleaves parathyroid hormone mRNA[review] PMID: 17565271
    19. FGF23 suppressed both parathyroid hormone (PTH) secretion and PTH gene expression PMID: 17992255
    20. IL-18 is regulated by parathyroid hormone and is required for its bone anabolic actions PMID: 18165223
    21. These data indicate a novel role for KSRP in parathyroid hormone gene expression. PMID: 18583400
    22. Phosphate overload and normal parathyroid hormone infusion induced phenotypic changes in vascular smooth muscle cells, as evidenced by upregulated aortic expression of Runx2. PMID: 19075196
    23. Regulation of PTH mRNA stability by the calcimimetic R568 and the phosphorus binder lanthanum carbonate in chronic kidney disease. PMID: 19129257
    24. without altering the gross chromatin structure, PTH stimulates acetylation of histones H3 and H4 via recruitment of p300 to Runx2 bound to the MMP-13 promoter, resulting in gene activation PMID: 19423655
    25. estrogens regulate PTH indirectly, possibly through FGF23 PMID: 19628670
    26. Parathyroid hormone (PTH) suppresses rat PTH/PTH-related protein receptor gene promoter PMID: 11554727

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Parathyroid hormone family
  • 组织特异性：
    Hypothalamus and parathyroid gland.
  • 数据库链接：

    KEGG: rno:24694

    STRING: 10116.ENSRNOP00000019199

    UniGene: Rn.9768