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  4. Rat Pancreatic prohormone(PPY) ELISA kit

Rat Pancreatic prohormone(PPY) ELISA kit

  • 中文名称：
    大鼠胰多肽酶联免疫试剂盒
  • 货号：
    CSB-E12747r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat PPY ELISA Kit was designed for the quantitative measurement of Rat PPY protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.78 pg/mL-50 pg/mL and the sensitivity is 0.2 pg/mL.

  • 缩写：
    PPY
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.78 pg/mL-50 pg/mL
  • 灵敏度：
    0.2 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 104    
    Range % 93-109    
    1:200 Average % 100    
    Range % 91-104    
    1:400 Average % 97    
    Range % 88-101    
    1:800 Average % 90    
    Range % 82-94    
  • 回收率：
    The recovery of rat PPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 83-94    
    EDTA plasma (n=4) 100 91-104    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    50 2.069 1.932 2.001 1.899    
    25 1.429 1.466 1.448 1.346    
    12.5 0.946 0.917 0.932 0.830    
    6.25 0.662 0.651 0.657 0.555    
    3.12 0.348 0.340 0.344 0.242    
    1.56 0.242 0.237 0.240 0.138    
    0.78 0.164 0.159 0.162 0.060    
    0 0.104 0.100 0.102 　    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    NPY family
  • 数据库链接：

    KEGG: rno:24677

    STRING: 10116.ENSRNOP00000028318

    UniGene: Rn.9755