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  4. Rat Pancreatic Lipase,PL ELISA Kit

Rat Pancreatic Lipase,PL ELISA Kit

  • 中文名称：
    大鼠胰脂肪酶(PL)酶联免疫试剂盒
  • 货号：
    CSB-E13784r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat PNLIP ELISA Kit was designed for the quantitative measurement of Rat PNLIP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 U/mL-20 U/mL and the sensitivity is 0.078 U/mL.

  • 别名：
    Pnlip ELISA Kit; Pancreatic triacylglycerol lipase ELISA Kit; PL ELISA Kit; Pancreatic lipase ELISA Kit; EC 3.1.1.3 ELISA Kit
  • 缩写：
    PNLIP
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.312 U/mL-20 U/mL
  • 灵敏度：
    0.078 U/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 106
    Range % 103-109
    1:2 Average % 96
    Range % 91-101
    1:4 Average % 92
    Range % 87-97
    1:8 Average % 95
    Range % 89-99
  • 回收率：
    The recovery of rat PL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 96-106
    EDTA plasma (n=4) 94 87-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    U/ml OD1 OD2 Average Corrected
    20 2.267 2.355 2.311 2.181
    10 1.848 1.855 1.852 1.722
    5 1.230 1.262 1.246 1.116
    2.5 0.712 0.744 0.728 0.598
    1.25 0.374 0.355 0.365 0.235
    0.625 0.289 0.287 0.288 0.158
    0.312 0.222 0.212 0.217 0.087
    0 0.126 0.133 0.130 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
  • 基因功能参考文献：
    1. Pnlip encoding pancreatic lipase is a possible candidate for intramuscular fat accumulation QTL in OLETF rat. PMID: 21469501
    2. Regulated expression of pancreatic triglyceride lipase after rat traumatic brain injury PMID: 19760487
    3. Data show that pancreatic lipase and pancreatic lipase related protein 2 gene expression is regulated anticoordinately by the amount of dietary polyunsaturated fat starting as early as the preweanling phase of development. PMID: 15181189
    4. Pnlip is possible candidate for the Nidd6/of QTL in the OLETF rat PMID: 15883013
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 组织特异性：
    Expressed by the pancreas. Pancreatic secretory (zymogen) granule.
  • 数据库链接：