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Rat P-Selectin ELISA kit

  • 中文名称：
    大鼠P选择素(P-Selectin/CD62P)酶联免疫试剂盒
  • 货号：
    CSB-E07399r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat P-Selectin ELISA kit is a powerful tool for researchers in the field of cardiovascular research. This ELISA kit allows for the quantitative measurement of P-selectin levels in a range of sample types from Rattus norvegicus (Rat), including serum, plasma, and tissue homogenates.

    With a detection range of 9.38 ng/mL to 600 ng/mL and a sensitivity of 2.34 ng/mL, this ELISA kit delivers accurate and precise results, making it an essential tool for inflammation, immunology and cardiovascular research. The assay time is only 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.

    The Rat P-Selectin ELISA kit utilizes a sandwich assay principle, where the P-selectin antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.

    This ELISA kit has been cited in over 10 publications, highlighting its reliability and utility in inflammation, immunology and cardiovascular research. It is a valuable addition to any research laboratory studying inflammation, immunology and cardiovascular research, providing accurate and reliable measurement of P-selectin levels in rat samples.

  • 别名：
    Selp; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD antigen CD62P
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    9.38 ng/mL-600 ng/mL
  • 灵敏度：
    2.34 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %85-99
    1:2Average %92
    Range %84-105
    1:4Average %97
    Range %92-103
    1:8Average %94
    Range %82-98
  • 回收率：
    The recovery of rat P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9795-100
    EDTA plasma (n=4)9592-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    6002.755 2.812 2.784 2.616
    3002.217 2.236 2.227 2.059
    1501.545 1.524 1.535 1.367
    751.051 0.979 1.015 0.847
    37.50.587 0.622 0.605 0.437
    18.750.456 0.438 0.447 0.279
    9.380.288 0.279 0.284 0.116
    00.173 0.162 0.168 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

Hello,
please let me know whether this elisa kit can be use to detect the P selectin which is released from platelet or not.

A:
Thanks for your inquiry.
Sorry we did not ever validate it before.
We suggest you do pre-testing first and determine it by yourself.

靶点详情

  • 功能：
    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.
  • 基因功能参考文献：
    1. Aortic cyclooxygenase-2 and P-selectin were significantly down-regulated in plant sterols and plant sterols + tripeptides groups. PMID: 22575043
    2. Fibrinogen can interact with P-selectin in order to contribute to the development of atherosclerosis, high levels of fibrinogen and P-selectin can be regarded as risk factors for markers of atherosclerosis. PMID: 22340239
    3. Renal injury after ischemia/reperfusion may relate to the strong expression of P-selectin. NO may have a protective affect by decreasing the expression of P-selectin and alleviating the adhesion, aggregation and infiltration of neutrophils. PMID: 21180132
    4. Both baicalin and octreotide can exert some protective effects on multiple organs of rats with severe acute pancreatitis via decreased P-selectin expression. PMID: 19780886
    5. Volatile oil of Magnolia biondii can protect the kidney in rats with diabetic nephropathy by inhibiting the expressions of P-selectin protein in serum and in renal tissue. PMID: 18471420
    6. P-selectin, contribute to massive infiltration of Mo into the interstitium by functioning as specialized post-capillary venules. PMID: 12081568
    7. P-selectin was highly expressed on glomerular endothelial cells after injection of nephrotoxic serum.P-selectin partially mediates glomerular infiltration of macrophage in experimental crescentic glomerulonephritis. PMID: 12100021
    8. Blockade of this molecule protects rat steatotic liver grafts from ischemia/reperfusion injury PMID: 12201360
    9. P-selectin increases CD45-positive leukocyte activation, inflammation and neointimal formation in a Zucker diabetic rat carotid artery balloon injury model. PMID: 12377736
    10. Neointimal rat SMCs supported a 2.5-fold higher arrest of monocytes and "memory" T lymphocytes than medial SMCs, which was dependent on both P-selectin and VLA-4. PMID: 14963004
    11. P-selectin increased significantly in hepatic sinusoidal endothelial cells and renal tubular epithelial cells 1 h after ischemia-reperfusion PMID: 15742404
    12. expression of the P-selectin gene is induced through the activation of NF-kappaB and that P-selectin may be involved in the pathogenesis of diabetic nephropathy PMID: 15888973
    13. P-selectin plays a role in ischemia-reperfusion injury in experimental myocardial infarct. PMID: 17488661

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Selectin/LECAM family
  • 组织特异性：
    Not detected in the absence of exposure to lipopolysaccharide (LPS). Detected only after exposure to lipopolysaccharide (LPS) in the tissues examined: spleen, lung, brain, liver, heart, kidney, thymus and small intestine.
  • 数据库链接：