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Rat Netrin-1(NTN1) ELISA kit

  • 中文名称：
    大鼠神经生长因子1(NTN1)酶联免疫试剂盒
  • 货号：
    CSB-EL016127RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat NTN1 ELISA Kit was designed for the quantitative measurement of Rat NTN1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

  • 别名：
    Ntn1 ELISA Kit; Netrin-1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    18.75 pg/mL-1200 pg/mL
  • 灵敏度：
    4.7 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LIPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 85    
    Range % 81-89    
    1:2 Average % 93    
    Range % 90-95    
    1:4 Average % 83    
    Range % 80-86    
    1:8 Average % 110    
    Range % 104-116    
  • 回收率：
    The recovery of mouse LIPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 89 85-97    
    EDTA plasma (n=4) 101 98-104    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    U/ml OD1 OD2 Average Corrected    
    10 2.115 2.201 2.158 2.025    
    5 1.658 1.638 1.648 1.515    
    2.5 1.182 1.201 1.192 1.059    
    1.25 0.745 0.738 0.742 0.609    
    0.625 0.418 0.431 0.425 0.292    
    0.312 0.325 0.319 0.322 0.189    
    0.156 0.204 0.211 0.208 0.075    
    0 0.134 0.132 0.133 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Involved in dorsal root ganglion axon projection towards the spinal cord. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis.
  • 亚细胞定位：
    Secreted. Cytoplasm.
  • 数据库链接：