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  4. Rat Matrix Metalloproteinase 14,MMP-14 ELISA Kit

Rat Matrix Metalloproteinase 14,MMP-14 ELISA Kit

  • 中文名称：
    大鼠基质金属蛋白酶14(MMP-14)酶联免疫试剂盒
  • 货号：
    CSB-E13158r
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat MMP14 ELISA Kit was designed for the quantitative measurement of Rat MMP14 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.

  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    46.88 pg/mL-3000 pg/mL
  • 灵敏度：
    11.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 88-95    
    1:2 Average % 95    
    Range % 90-102    
    1:4 Average % 97    
    Range % 95-107    
    1:8 Average % 101    
    Range % 96-105    
  • 回收率：
    The recovery of rat MMP-14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 87-96    
    EDTA plasma (n=4) 98 95-103    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    3000 2.288 2.289 2.289 2.092    
    1500 1.736 1.752 1.744 1.547    
    750 1.118 1.097 1.108 0.911    
    375 0.674 0.727 0.701 0.504    
    187.5 0.455 0.433 0.444 0.247    
    93.75 0.336 0.331 0.334 0.137    
    46.88 0.265 0.256 0.261 0.064    
    0 0.205 0.189 0.197 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15 in association with pro-MMP2. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis.
  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：