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  4. Rat Mast Cell Tryptase,MCT ELISA Kit

Rat Mast Cell Tryptase,MCT ELISA Kit

  • 中文名称：
    大鼠肥大细胞类胰蛋白酶(MCT)酶联免疫试剂盒
  • 货号：
    CSB-E13627r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat TPSAB1 ELISA Kit was designed for the quantitative measurement of Rat TPSAB1 protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    Tpsab1 ELISA Kit; Mcp7 ELISA Kit; Mcpt7 ELISA Kit; Tpsb1 ELISA Kit; Tryptase ELISA Kit; EC 3.4.21.59 ELISA Kit; Mast cell protease 7 ELISA Kit; rMCP-7 ELISA Kit; Tryptase alpha/beta-1 ELISA Kit; Tryptase ELISA Kit; skin ELISA Kit
  • 缩写：
    TPSAB1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, urine
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %90-92
    1:2Average %92
    Range %87-96
    1:4Average %94
    Range %89-97
    1:8Average %87
    Range %82-92
  • 回收率：
    The recovery of rat MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-102
    EDTA plasma (n=4)104100-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.464 2.489 2.477 2.326
    201.823 1.863 1.843 1.692
    101.229 1.263 1.246 1.095
    50.812 0.847 0.830 0.679
    2.50.482 0.498 0.490 0.339
    1.250.312 0.339 0.326 0.175
    0.6250.241 0.258 0.250 0.099
    00.150 0.152 0.151 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
  • 亚细胞定位：
    Secreted. Note=Released from the secretory granules upon mast cell activation.
  • 蛋白家族：
    Peptidase S1 family, Tryptase subfamily
  • 组织特异性：
    Mast cells.
  • 数据库链接：