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  4. Rat Lecithin Cholesterol Acyltransferase(LCAT) ELISA Kit

Rat Lecithin Cholesterol Acyltransferase(LCAT) ELISA Kit

  • 中文名称：
    大鼠卵磷脂胆固醇酰基转移酶(LCAT)酶联免疫试剂盒
  • 货号：
    CSB-E14201r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat LCAT ELISA Kit was designed for the quantitative measurement of Rat LCAT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.87 ng/mL-120 ng/mL and the sensitivity is 0.94 ng/mL.

  • 别名：
    LcatPhosphatidylcholine-sterol acyltransferase ELISA kit; EC 2.3.1.43 ELISA kit; Lecithin-cholesterol acyltransferase ELISA kit; Phospholipid-cholesterol acyltransferase ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.87 ng/mL-120 ng/mL
  • 灵敏度：
    0.94 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LCAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %102
    Range %92-107
    1:200Average %88
    Range %84-93
    1:400Average %101
    Range %94-105
    1:800Average %102
    Range %92-105
  • 回收率：
    The recovery of rat LCAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-96
    EDTA plasma (n=4)10292-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    1200.075 0.079 0.077
    600.172 0.178 0.175
    300.374 0.382 0.378
    150.634 0.624 0.629
    7.51.178 1.146 1.162
    3.751.504 1.533 1.519
    1.871.673 1.622 1.648
    02.155 2.078 2.117
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF. Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 组织特异性：
    Detected in blood plasma (at protein level).
  • 数据库链接：