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Rat Interleukin 5,IL-5 ELISA KIT

  • 中文名称:
    大鼠白介素5(IL-5)酶联免疫试剂盒
  • 货号:
    CSB-E07435r
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Rat IL5 ELISA Kit was designed for the quantitative measurement of Rat IL5 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

  • 别名:
    Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    15.6 pg/mL-1000 pg/mL
  • 灵敏度:
    3.9 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %85
    Range %82-91
    1:2Average %92
    Range %83-99
    1:4Average %101
    Range %93-107
    1:8Average %96
    Range %90-104
  • 回收率:
    The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8885-94
    EDTA plasma (n=4)9490-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.407 2.457 2.432 2.268
    5001.768 1.736 1.752 1.588
    2501.065 1.098 1.082 0.918
    1250.704 0.735 0.720 0.556
    62.50.448 0.469 0.459 0.295
    31.20.323 0.300 0.312 0.148
    15.60.221 0.229 0.225 0.061
    00.161 0.167 0.164  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    IL-5 family
  • 数据库链接: