Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat Interleukin 21,IL-21 ELISA Kit

Rat Interleukin 21,IL-21 ELISA Kit

  • 中文名称：
    大鼠白介素21(IL-21)酶联免疫试剂盒
  • 货号：
    CSB-E11843r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat IL21 ELISA Kit was designed for the quantitative measurement of Rat IL21 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Il21Interleukin-21 ELISA Kit; IL-21 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %85-96
    1:2Average %89
    Range %84-95
    1:4Average %103
    Range %99-108
    1:8Average %99
    Range %94-105
  • 回收率：
    The recovery of rat IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-101
    EDTA plasma (n=4)9288-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.601 2.544 2.573 2.456
    5001.921 1.952 1.937 1.820
    2501.198 1.122 1.160 1.043
    1250.735 0.709 0.722 0.605
    62.50.425 0.459 0.442 0.325
    31.20.272 0.257 0.265 0.148
    15.60.181 0.172 0.177 0.060
    00.116 0.118 0.117
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-15/IL-21 family
  • 数据库链接：