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  4. Rat Interleukin 16,IL-16 ELISA KIT

Rat Interleukin 16,IL-16 ELISA KIT

  • 中文名称：
    大鼠白介素16(IL-16)酶联免疫试剂盒
  • 货号：
    CSB-E07452r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    The rat IL16 Elisa kit is suitable for the quantitative measurement of rat IL16 in serum, plasma, or tissue homogenates. This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of rat IL16 in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

    IL16, formerly known as lymphocyte chemoattractant factor, serves as a proinflammatory factor chemotactic for immune cells, including T cells, monocytes, and eosinophils. As a pro-inflammatory cytokine, IL-16 plays a pivotal role in initiating innate and adaptive immune responses and assists in generating a local inflammatory response in vertebrates. It acts through CD4 as its signal-transducing receptor and is upregulated in allergic responses in nasal tissues and bronchoalveolar lavage fluid of histamine-challenged asthma patients. IL16 plays a key role in inflammatory diseases as well as in tumorigenesis of osteosarcoma (OS).

  • 缩写：
    IL-16
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 87-96  
    1:2 Average % 98  
    Range % 94-102  
    1:4 Average % 103  
    Range % 98-107  
    1:8 Average % 89  
    Range % 84-96  
  • 回收率：
    The recovery of rat IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 88 82-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.757 2.656 2.707 2.524  
    1000 2.223 2.122 2.173 1.990  
    500 1.488 1.467 1.478 1.295  
    250 0.937 0.926 0.932 0.749  
    125 0.589 0.578 0.584 0.401  
    62.5 0.425 0.414 0.420 0.237  
    31.25 0.305 0.297 0.301 0.118  
    0 0.183 0.182 0.183 　  
  • 数据处理：
  • 货期：
    3-5 working days

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