Rat Insulin-like Growth Factors 1 Receptor,IGF-1R ELISA Kit

Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R. When present in a hybrid receptor with INSR, binds IGF1.

1. overexpression of decorin ameliorated diabetic cardiomyopathy and promoted angiogenesis through the IGF1R-AKT-VEGF signaling pathway in vivo and in vitro. PMID: 28290552
2. Mechanical stretch regulated L6 myoblasts proliferation, which may be mediated by the changes in PI3K/Akt and MAPK activations regulated by IGF-1R, despite no detectable IGF-1 from stretched L6 myoblasts. PMID: 29865254
3. Our results suggested that miR-223 may suppress proliferation of cortical neurons that were treated with oxygen-glucose deprivation and simulated reperfusion via inhibiting IGF1R expression. PMID: 29663740
4. IGF1R signaling is not involved in resistance exercise-induced cardiac hypertrophy. PMID: 28937244
5. IgA enhances the IGF-1 activity in Glomerular Mesangial Cells via stimulation of IGF-1receptor gene transcription and suggest a role for IGF-1 in pathogenesis of IgA-Induced Nephropathy. PMID: 28668953
6. The study showed that cortical and hippocampal IGF-1R expression were altered in response to traumatic stress, and after a prolonged exposure therapy. PMID: 28438613
7. An age-dependent reduction of IGF-1R expression was found in the paraventricular nucleus in a model of inflammation. PMID: 27626839
8. These results demonstrated that MeCP2 silencing of H19 can alter the IGF1R overexpression, thus contributing to hepatic stellate cell proliferation and could suggest the development of combination therapies that target the MeCP2. PMID: 27350269
9. Seminal vesicles were evaluated by morphological and immunohistochemical parameters; androgenic receptor (AR), Insulin-like growth factor 1 (IGFR-1) and metalloproteinase 9 (MMP-9). Intense AR reactivity was seen in both stroma and epithelial regions in the TRAMP 22 group. Intense IGFR-1 and MMP-9 stromal immunolabeling was identified in both TRAMP groups PMID: 27036326
10. IGF-1 action in the brain and periphery provides a 'balance' between its beneficial and detrimental actions PMID: 26534869
11. vimentin may be a novel ligand of IGF1R that promotes axonal growth in a similar manner to IGF1. PMID: 26170015
12. Data suggest that stimulation of IGF type 1 receptor-mediated signal transduction in endothelial blood-brain barrier of aging rats may contribute to improved endothelial blood-brain barrier function following ischemic stroke. PMID: 26556536
13. These findings suggest that down-regulation of miR-223 promotes degranulation via the PI3K/Akt pathway by targeting IGF-1R in mast cells. PMID: 25875646
14. Results indicate that increased IGF-1 levels after recurrent hippocampal neuronal firings might, in turn, promote seizure activity via IGF-1R-dependent mechanisms. PMID: 26286172
15. Up-regulation of TGFbeta1 and nitration of IGF-R1 by peroxynitrite are the likely contributors to impaired alveologenesis and chronic lung injury in the neonatal rat. PMID: 25514442
16. PYPE treatment increased protein and mRNA expression levels of activator protein1, which regulates cell proliferation and survival PMID: 25500890
17. miR-194 has a role in modulating burn-induced hyperglycemia via suppressing the expression of IGF-IR PMID: 25186839
18. Resveratrol effectively inhibits collagen I synthesis in IGF-1-stimulated colonic fibroblasts, partly by inhibiting IGF-1R activation, and SIRT1 is also responsible for the process. PMID: 24782617
19. The aim of this study was to immunohistochemically determine the distinct regional distribution pattern of IGF-1 receptor (IGF-IR) expression in various portions of newborn rat hippocampus with comparison between male/female and right/left hippocampi. PMID: 24287499
20. Data suggest age-related changes in hypothalamo-pituitary axis responsiveness via NMDA/NMDA receptor and kisspeptin/kisspeptin receptor signaling reflect reduced Igf1/Igf1 receptor signaling with aging of neuroendocrine cells of hypothalamus. PMID: 24617524
21. The blockade of IGF-IR with JB1 prevented the depolarising effects of both insulin and IGF-I on membrane potential. PMID: 24530896
22. IGF-IR expression may participate in rat hepatocarcinogenesis and its abnormality should be an early marker for hepatocytes malignant transformation. PMID: 24106410
23. Following exercise training, carciac IGFI-R was significantly increased in experimental diabetes. PMID: 22341695
24. diabetes during pregnancy strongly influences the regulation of IGF-1R in the developing cerebellum PMID: 23397157
25. results suggest that IGF-IR and ER signaling pathways might be involved in the protective effect of Rg1 against Abeta25-35-induced toxicity in PC12 cells PMID: 23603302
26. These findings indicate that there are prominent sexual differences in InsR and IGF-1R expression in the developing rat cerebellum. PMID: 23322319
27. these data demonstrate that axonal regeneration in adult CNS neurons requires re-expression and activation of IGF-1R, and targeting this system may offer new therapeutic approaches to enhancing axonal regeneration following trauma. PMID: 23349896
28. the IGF/IGF-R1 pathway does regulate alveologenesis, but as a catalyst of lung cell DNA synthesis, rather than as a specific effector of secondary crest formation. PMID: 23457189
29. Diabetes during pregnancy strongly influences the regulation of both IGF-1R and InsR in the right/left developing hippocampi. PMID: 22241286
30. Abeta(1-42) induced altered expression of IGF-I receptors in rat hippocampal cells. PMID: 22781651
31. Insulin-like growth factor 1 triggers a fast and independent nuclear calcium (Ca2+) signal in neonatal rat cardiac myocytes, human embryonic cardiac myocytes, and adult rat cardiac myocytes. PMID: 23118311
32. miR-153 and miR-223 are reduced in venous smooth muscle cells (VSMCs) by stretch stress, contributing to IGF-1R activation and resultant VSMC proliferation. PMID: 23046980
33. investigated the expression differences of insulin-like growth factor-1 (IGF1), IGF type 1 receptor (IGFR1) and IGF-binding protein-3 (IGFBP3) in mandibular condylar cartilage between male and female rats with experimentally created malocclusion PMID: 22758598
34. investigation into role of IGF-I receptor in cell proliferation: Data suggest that L-ascorbic acid (and a long-lasting analog) induces DNA synthesis and cell proliferation in primary hepatocytes by interacting, at least in part, with IGF-I receptor. PMID: 22429571
35. IGF-1/IGF-1R signaling system may involved in the onset and development of vascular dementia PMID: 22342912
36. Our study identifies miR-378 as a new cardioabundant microRNA that targets IGF1R. PMID: 22367207
37. Igf1r+/CD34+ immature ICC are putative adult progenitor cells, identified ultrastructurally as fibroblast-like ICC in Ws/Ws rat colon PMID: 19220583
38. IGF1R expression and activity via osteoprotegerin can modulate vascular smooth muscle cell calcification. PMID: 21447702
39. We identified upregulation of the IR/IGF-IR pathways as the earliest molecular events in N-nitrosomorpholine induced hepatocarcinogenesis. PMID: 21411721
40. c-kitpos GATA-4 high cCSCs exert a paracrine survival effect on cardiomyocytes through induction of the IGF-1R and signalling pathway PMID: 21179204
41. The excessive alcohol consumption caused morphological and molecular changes in the coagulating gland, characterizing the inverse relation of AR and IGFR-1 localization. PMID: 20605618
42. Data show that chromium was able to increase the basal and insulin-stimulated levels of IGF-1, IGF-1R, and ubiquitin deposition in skeletal muscle cells. PMID: 20013160
43. HG decreases expression of IGF-1R and decreases the association of acetylated histone-4 with the IGF-1R promoter PMID: 20633551
44. Downregulation of IGF-1R and IGF-2R gene expression may interfere with normal alveologenesis causing pulmonary hypoplasia in the nitrofen-induced CDH model. PMID: 20620343
45. desialylation of both IR and IGF-1R by Neu1 controls the net proliferative response of skeletal myoblasts to insulin PMID: 20100694
46. IGF-1/IGF1R induces Wnt4 expression and beta-catenin activation and also stimulates growth plate chondrocyte proliferation and modestly promotes chondrocyte hypertrophy PMID: 20200966
47. Data show that astroglial cells are required to upregulate the expression of IGF1-R in neurons during in vitro senescence, and suggest that astrocytes could play an important role in aging and age-related pathological processes. PMID: 19862643
48. Mechanotransduction in osteoblastic cells involves strain-regulated ESRalpha-mediated control of IGFI receptor sensitivity to Ambient IGF, leading to PI3-kinase/AKT-dependent Wnt/LRP5 receptor-independent activation of beta-catenin signaling PMID: 20042609
49. IGF-I receptor signaling is necessary for GnRH neuron activation under estrogen-positive feedback conditions. PMID: 20097715
50. localisation of immunoreactive IGF-I, IGFR, GH and GHR proteins in ovarian sections of 10-day-old rats PMID: 11964096

显示更多

收起更多

Cell membrane; Single-pass type I membrane protein.

Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily

KEGG: rno:25718

STRING: 10116.ENSRNOP00000019267

UniGene: Rn.10957