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  4. Rat Insulin-Like 3 (INSL3) ELISA Kit

Rat Insulin-Like 3 (INSL3) ELISA Kit

  • 中文名称：
    大鼠胰岛素样因子3(INSL3)酶联免疫试剂盒
  • 货号：
    CSB-E15850r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat INSL3 ELISA Kit was designed for the quantitative measurement of Rat INSL3 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    Insl3 ELISA Kit; Rlf ELISA Kit; Insulin-like 3 ELISA Kit; Leydig insulin-like peptide ELISA Kit; Ley-I-L ELISA Kit; Relaxin-like factor) [Cleaved into: Insulin-like 3 B chain; Insulin-like 3 A chain] ELISA Kit
  • 缩写：
    INSL3
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat INSL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 105
    Range % 101-109
    1:2 Average % 97
    Range % 94-99
    1:4 Average % 88
    Range % 83-96
    1:8 Average % 93
    Range % 89-98
  • 回收率：
    The recovery of rat INSL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 100 99-105
    EDTA plasma (n=4) 92 85-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 1.978 1.944 1.961 1.759
    5 1.436 1.545 1.491 1.289
    2.5 1.021 1.006 1.014 0.812
    1.25 0.665 0.645 0.655 0.453
    0.625 0.423 0.412 0.418 0.216
    0.312 0.312 0.325 0.319 0.117
    0.156 0.255 0.270 0.263 0.061
    0 0.198 0.205 0.202 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.
  • 基因功能参考文献：
    1. The observed postnatal difference in gubernacular malformations between two rate strains is due to different levels of the insl3 receptor differ among strains. PMID: 21633115
    2. data suggest that INSL3 signaling in fetal gubernaculum induces morphogenetic programs in male genitalia; investigation of differences in gene expression regulation by INSL3 in gubernaculum of male and female genitalia PMID: 20631401
    3. Demonstration of INSL3 as the ligand for LGR8 facilitates understanding of the mechanism of testis descent and allows studies on the role of INSL3 in gonadal and other physiological processes PMID: 12114498
    4. Rat leucine-rich repeat-containing G protein-coupled receptor 8 bound INSL3 with high affinity, clearly demonstrating that it is the rat INSL3 receptor PMID: 15956681
    5. INSL3/leucine-rich repeat-containing G protein-coupled receptor 8 signaling may be involved in the genesis and/or developmental maturation of renal glomeruli PMID: 15956754
    6. Prenatal mono-n-butyl phthlate may inhibit the INSL3 gene expression associated with the transabdominal testicular ascent in fetal rats, thereby causing undescended testis in postnatal offspring PMID: 16338306
    7. In utero exposure to flutamide decreases expression levels of Insl3 particularly at 15 days post conception. PMID: 18772241
    8. INSL3 evidently traverses the blood-testis barrier to enter the seminiferous compartment, rete testis, and epididymis in sufficient concentration to be able to address the specific INSL3 receptors (RXFP2) on post-meiotic germ cells and in the epididymis. PMID: 19420383
    9. Prenatal exposure to diaethylstilbestrol can inhibit transabdominal testicular descent via down-regulating the expression of INSL3, which is induced by down-regulating the expression of SF-1. PMID: 19493424

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 组织特异性：
    Expressed in Leydig cells of the testis, and weakly in the theca interna cells of antral follicles and the corpus luteum of the ovary.
  • 数据库链接：