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  4. Rat Hemoglobin (Hb) ELISA Kit

Rat Hemoglobin (Hb) ELISA Kit

  • 中文名称：
    大鼠血红蛋白酶联免疫试剂盒
  • 货号：
    CSB-E13036r
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    The rat hemoglobin (Hb) ELISA Kit is engineered for accurate measurement of rat hemoglobin levels from samples including serum, plasma, or lysate for RBC. It uses the Competitive-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the rat hemoglobin content in the sample. The color intensity is negatively correlated with hemoglobin content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

    Hemoglobin is a two-way respiratory carrier, transporting oxygen from the lungs to the tissues and facilitating the return transport of carbon dioxide. In arterial circulation, hemoglobin has a high affinity for oxygen and a low affinity for carbon dioxide, organic phosphates, and hydrogen and chloride ions. In addition to being a molecular heat transducer through its oxygenation-deoxygenation cycle as well as a modulator of erythrocyte metabolism, hemoglobin's oxidation is an onset of erythrocyte senescence.It also mediates reactive oxygen and nitrogen species detoxification.p>

  • 别名：
    Hba1 ELISA Kit; Hba-a1 ELISA Kit; Hemoglobin subunit alpha-1/2 ELISA Kit; Alpha-1/2-globin ELISA Kit; Hemoglobin alpha-1/2 chain ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, lysate for RBC.
  • 检测范围：
    0.78 μg/mL-50 μg/mL
  • 灵敏度：
    0.39 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Hb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:5 Average % 101    
    Range % 95-107    
    1:10 Average % 93    
    Range % 89-97    
    1:20 Average % 95    
    Range % 90-100    
    1:40 Average % 90    
    Range % 85-97    
  • 回收率：
    The recovery of rat Hb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 89 84-93    
    EDTA plasma (n=4) 92 88-96    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    µg/ml OD1 OD2 Average      
    50 0.169 0.176 0.1725      
    25 0.292 0.311 0.3015      
    12.5 0.486 0.483 0.4845      
    6.25 0.819 0.795 0.807      
    3.12 1.373 1.347 1.36      
    1.56 2.013 2.024 2.0185      
    0.78 2.461 2.493 2.477      
    0 2.721 2.652 2.6865      
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in oxygen transport from the lung to the various peripheral tissues.
  • 基因功能参考文献：
    1. Results report that hemoglobin chains alpha2 nad beta are expressed in mammalian brain neurons and are regulated by a mitochondrial toxin. PMID: 19479992
  • 蛋白家族：
    Globin family
  • 组织特异性：
    Red blood cells.
  • 数据库链接：