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Rat Gamma-glutamyltranspeptidase 1(GGT1) ELISA kit

  • 中文名称：
    大鼠γ谷氨酰转肽酶1(GGT1)酶联免疫试剂盒
  • 货号：
    CSB-EL009394RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat GGT1 ELISA Kit was designed for the quantitative measurement of Rat GGT1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 mIU/mL-300 mIU/mL and the sensitivity is 1.2 mIU/mL.

  • 别名：
    Ggt1; Ggt; Glutathione hydrolase 1 proenzyme; Gamma-glutamyltransferase 1; Gamma-glutamyltranspeptidase 1; GGT 1; Leukotriene-C4 hydrolase; CD antigen CD224
  • 缩写：
    GGT1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    4.7 mIU/mL-300 mIU/mL
  • 灵敏度：
    1.2 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GGT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-103  
    1:2 Average % 90  
    Range % 85-94  
    1:4 Average % 92  
    Range % 88-99  
    1:8 Average % 89  
    Range % 84-93  
  • 回收率：
    The recovery of rat GGT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    300 2.379 2.311 2.345 2.252  
    150 1.681 1.726 1.704 1.611  
    75 1.205 1.224 1.215 1.122  
    37.5 0.704 0.726 0.715 0.622  
    18.8 0.342 0.317 0.330 0.237  
    9.4 0.210 0.199 0.205 0.112  
    4.7 0.125 0.118 0.122 0.029  
    0 0.090 0.095 0.093    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates and other gamma-glutamyl compounds, such as leukotriene C4 (LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.
  • 基因功能参考文献：
    1. an extensive ductular reaction in advanced Hepatocellular carcinoma (HCC) and a strong correlation between PCK7 and levels of gammaGT and the poor prognosis and aggressiveness of HCC, is reported. PMID: 27525410
    2. These data demonstrate the important role of endothelial GGT activity in mediating the vasorelaxant effect of S-nitrosoglutathione in rat aorta . PMID: 22984412
    3. The expression of gamma-glutamyltransferase in rat colon carcinoma cells is distinctly regulated during differentiation and oxidative stress PMID: 12030384
    4. Radiation of CC531 colon carcinoma cells blocked cell proliferation, increased cellular size, initiated apoptosis and upregulated GGT activity and protein levels in a dose- and time-related manner. PMID: 16386375
    5. With the co-existence of inflammation and hypoxia in the brain after ischemic stroke, dynamic changes of gammaGT activity reflect evolving changes of endothelial function. PMID: 16973162
    6. Data suggest that extracellular glutathione elicits gamma-glutamyl transpeptidase and ROS-dependent transactivation of tyrosine kinase signaling that upregulates potassium channel activity or expression via redox-mediated mechanisms. PMID: 19419996
    7. The acylation reaction of GGT by gamma-glutamyl anilides can apparently be accelerated by either electron-withdrawing or electron-donating substituents, suggesting a concerted mechanism involving kinetic general acid catalysis of aniline departure. PMID: 11601992

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Gamma-glutamyltransferase family
  • 组织特异性：
    Detected in adult kidney and mammary gland, and in fetal liver.
  • 数据库链接：

    KEGG: rno:116568

    UniGene: Rn.10010