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  4. Rat Dehydroepiandrosterone,DHEA ELISA Kit

Rat Dehydroepiandrosterone,DHEA ELISA Kit

  • 中文名称：
    大鼠脱氢表雄酮(DHEA)酶联免疫试剂盒
  • 货号：
    CSB-E08227r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat DHEA ELISA Kit was designed for the quantitative measurement of Rat DHEA protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.05 ng/mL-10 ng/mL and the sensitivity is 0.025 ng/mL .

  • 缩写：
    DHEA
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.05 ng/mL-10 ng/mL
  • 灵敏度：
    0.025 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %102
    Range %98-107
    1:2Average %92
    Range %88-96
    1:4Average %89
    Range %85-93
    1:8Average %100
    Range %97-104
  • 回收率：
    The recovery of rat DHEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-92
    EDTA plasma (n=4)9488-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1OD2Average
    100.162 0.158 0.160
    2.50.307 0.298 0.303
    0.80.487 0.504 0.496
    0.20.801 0.810 0.806
    0.051.224 1.305 1.265
    02.000 1.899 1.950
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Please provide the following information regarding Rat Dehydroepiandrosterone, DHEA ELISA Kit (CSB-E08227r): What is the active ingredient of the stop solution and its concentration?
What is the type, concentration, and state (liquid/lyophilized) of the preservatives used?
What is the expression host of the standard?
which components have H202 and the concentration of H202 in them?Just to confirm this I am inquiring for 24 and 96 well of this kit. Please confirm if the information will be the same for both the 24T and 96T in regards to Rat Dehydroepiandrosterone, DHEA ELISA Kit (CSB-E08227r)?
**if the preservative/s are not liquid, what is the concentration of the component prior to reconstitution (while still lyophilized)?

A:
Thanks for your inquiry.
CSB-E08227r
Standard is purified DHEA.
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000 dilution）.
Sorry we can not disclose which components have H202 and the concentration of H202 in them.
For 24T and 96T, the component information is the same. Only the volume of reagents is different.
Pls let me know if you have any further questions. Thank you.
Q:

Can you please clarify if the preservative is liquid or lyophilized?
If it's lyophilized, please let us know concentration too.

A:
Thanks for your further inquiry. It is liquid.