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Rat Cytochrome P450 19A1(CYP19A1) ELISA kit

  • 中文名称:
    大鼠细胞色素P450 19A1(CYP19A1)酶联免疫试剂盒
  • 货号:
    CSB-EL006394RA
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Rat CYP19A1 ELISA Kit was designed for the quantitative measurement of Rat CYP19A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.

  • 别名:
    Cyp19a1 ELISA Kit; Arom ELISA Kit; Cyp19Aromatase ELISA Kit; EC 1.14.14.14 ELISA Kit; CYPXIX ELISA Kit; Cytochrome P-450AROM ELISA Kit; Cytochrome P450 19A1 ELISA Kit; Estrogen synthase ELISA Kit
  • 缩写:
    CYP19A1
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    18.75 pg/mL-1200 pg/mL
  • 灵敏度:
    4.68 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CYP19A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %101
    Range %95-107
    1:2Average %91
    Range %86-97
    1:4Average %88
    Range %84-92
    1:8Average %95
    Range %90-100
  • 回收率:
    The recovery of rat CYP19A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8580-90
    EDTA plasma (n=4)9691-103
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1OD2AverageCorrected
    12002.452 2.272 2.362 2.237
    6001.705 1.832 1.769 1.644
    3001.286 1.316 1.301 1.176
    1500.829 0.880 0.855 0.730
    750.504 0.469 0.487 0.362
    37.50.301 0.285 0.293 0.168
    18.750.203 0.211 0.207 0.082
    00.127 0.122 0.125
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • 亚细胞定位:
    Membrane; Peripheral membrane protein.
  • 蛋白家族:
    Cytochrome P450 family
  • 数据库链接: