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  4. Rat Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Rat Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

  • 中文名称：
    大鼠肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒
  • 货号：
    CSB-E14403r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        CK-MB (肌酸激酶同工酶-MB，Creatine Kinase-MB) 是一种肌酸激酶同工酶，主要分布于心肌细胞中，是一种心肌标志物，用于心肌梗死的诊断。CK-MB可以随着心肌细胞受损而释放到血液中，检测血清中CK-MB的水平可以帮助医生判断心肌梗死的程度。
        j9九游会登录入口首页生物所提供的Rat Creatine Kinase MB isoenzyme,CK-MB ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测大鼠血清、尿液、组织匀浆、血浆样本中的CK-MB，其灵敏度为0.078 ng/mL，检测范围为0.312 ng/mL-20 ng/mL。
     
  • 别名：
    N/A
  • 缩写：
    CK-MB
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, urine, tissue homogenates, plasma
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 98-103  
    1:2 Average % 96  
    Range % 92-100  
    1:4 Average % 92  
    Range % 87-98  
    1:8 Average % 89  
    Range % 84-94  
  • 回收率：
    The recovery of rat CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 96-104  
    EDTA plasma (n=4) 93 89-97  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.646 2.687 2.667 2.564  
    10 1.834 1.856 1.845 1.742  
    5 1.096 1.099 1.098 0.995  
    2.5 0.593 0.583 0.588 0.485  
    1.25 0.356 0.368 0.362 0.259  
    0.625 0.229 0.249 0.239 0.136  
    0.312 0.165 0.174 0.170 0.067  
    0 0.101 0.105 0.103 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat CK-MB antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled CK-MB antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

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靶点详情

  • 最新研究进展：
    CK-MB是一种心肌特异性蛋白质，是临床上常用的心肌损伤指标之一。CK-MB可以用于诊断急性心肌梗死等心血管疾病。近年来，研究人员发现CK-MB在许多其他生理和病理过程中也发挥重要作用，如运动训练、重症肌无力等。