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  4. Rat Complement 4,C4 ELISA Kit

Rat Complement 4,C4 ELISA Kit

  • 中文名称：
    大鼠补体蛋白4(C4)酶联免疫试剂盒
  • 货号：
    CSB-E08706r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Complement 4 (C4) ELISA Kit was designed for the quantitative measurement of Rat Complement 4 (C4) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

  • 别名：
    C4 ELISA Kit; C4aComplement C4 [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain] ELISA Kit
  • 缩写：
    C4
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 ng/mL-800 ng/mL
  • 灵敏度：
    3.12 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %99
    Range %96-102
    1:400Average %87
    Range %82-93
    1:800Average %100
    Range %96-104
    1:1600Average %84
    Range %81-89
  • 回收率：
    The recovery of rat C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9390-96
    EDTA plasma (n=4)8884-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    8002.604 2.658 2.631 2.540
    4002.185 2.198 2.192 2.101
    2001.673 1.698 1.686 1.595
    1001.069 1.097 1.083 0.992
    500.626 0.636 0.631 0.540
    250.292 0.271 0.282 0.191
    12.50.188 0.180 0.184 0.093
    00.090 0.092 0.091
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes.; Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory processes. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • 基因功能参考文献：
    1. Our study provides the first evidence that sustained selective EPAC activation leads to an increase in TRPC3 and C4 protein expression and induces a proarrhythmic SOCE-like activity in adult rat ventricular cardiomyocytes PMID: 26219954
    2. The expression of the complement component C3f and complement component 4A have potential for use in post-transplant diagnosis of rejection. PMID: 23613499
    3. This study presents the genomic organization of the two C4 gene-containing modules and the expression of the two C4 genes in the rat (Rattus norvegicus). PMID: 15634920
  • 亚细胞定位：
    Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite.
  • 数据库链接：