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  4. Rat Coagulation factor VII(F7) ELISA kit

Rat Coagulation factor VII(F7) ELISA kit

  • 中文名称：
    大鼠凝血因子VII(F7)酶联免疫试剂盒
  • 货号：
    CSB-EL007930RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Coagulation factor VII (F7) ELISA Kit was designed for the quantitative measurement of Rat Coagulation factor VII (F7) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

  • 别名：
    F7Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Blood Coagulation
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:200 Average % 91
    Range % 86-98
    1:400 Average % 97
    Range % 92-106
    1:800 Average % 93
    Range % 88-99
    1:1600 Average % 89
    Range % 83-95
  • 回收率：
    The recovery of rat F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 87-97
    EDTA plasma (n=4) 109 103-115
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    50 2.468 2.652 2.560 2.424
    25 2.115 2.076 2.096 1.960
    12.5 1.503 1.504 1.504 1.368
    6.25 0.845 0.805 0.825 0.689
    3.12 0.526 0.524 0.525 0.389
    1.56 0.381 0.359 0.370 0.234
    0.78 0.274 0.259 0.267 0.131
    0 0.137 0.135 0.136 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
  • 基因功能参考文献：
    1. The EGF(1) region of rat coagulation factor can mediate the specific binding of FVII with tissue factor. PMID: 18315926
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Plasma.
  • 数据库链接：