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  4. Rat Caspase-9,Casp-9 ELISA Kit

Rat Caspase-9,Casp-9 ELISA Kit

  • 中文名称：
    大鼠胱天蛋白酶9(Casp-9)酶联免疫试剂盒
  • 货号：
    CSB-E08863r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Casp-9 ELISA Kit was designed for the quantitative measurement of Rat Casp-9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 缩写：
    Casp-9
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %90-99
    1:2Average %95
    Range %91-102
    1:4Average %98
    Range %94-103
    1:8Average %102
    Range %96-106
  • 回收率：
    The recovery of rat Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)10096-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40001.846 1.946 1.896 1.752
    20001.442 1.542 1.492 1.348
    10000.986 1.016 1.001 0.857
    5000.546 0.556 0.551 0.407
    2500.320 0.330 0.325 0.181
    1250.266 0.278 0.272 0.128
    62.50.204 0.207 0.206 0.062
    00.143 0.144 0.144 　
  • 数据处理：
  • 货期：
    3-5 working days

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