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Rat Caspase-1(CASP1) ELISA kit

  • 中文名称：
    大鼠半胱氨酸天冬氨酸蛋白酶1(CASP1)酶联免疫试剂盒
  • 货号：
    CSB-EL004543RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat CASP1 ELISA Kit was designed for the quantitative measurement of Rat CASP1 protein in serum, plasma, cell lysates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

  • 别名：
    Casp1 ELISA kit; Il1bc ELISA kit; Il1bceCaspase-1 ELISA kit; CASP-1 ELISA kit; EC 3.4.22.36 ELISA kit; Interleukin-1 beta convertase ELISA kit; IL-1BC ELISA kit; Interleukin-1 beta-converting enzyme ELISA kit; ICE ELISA kit; IL-1 beta-converting enzyme ELISA kit; p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10] ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell lysates, cell culture supernates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CASP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 88-93    
    1:2 Average % 95    
    Range % 90-102    
    1:4 Average % 97    
    Range % 94-101    
    1:8 Average % 94    
    Range % 91-98    
  • 回收率：
    The recovery of rat CASP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 86-94    
    EDTA plasma (n=4) 99 93-105    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    400 2.189 2.321 2.255 2.047    
    200 1.729 1.754 1.742 1.534    
    100 1.151 1.165 1.158 0.950    
    50 0.755 0.748 0.752 0.544    
    25 0.456 0.468 0.462 0.254    
    12.5 0.317 0.325 0.321 0.113    
    6.25 0.277 0.290 0.284 0.076    
    0 0.201 0.215 0.208 　    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly.
  • 亚细胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Peptidase C14A family
  • 数据库链接：