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Rat Calcitonin,CT ELISA Kit

  • 中文名称:
    大鼠降钙素(CT)酶联免疫试剂盒
  • 货号:
    CSB-E05132r
  • 规格:
    96T/48T
  • 价格:
    ¥3900/¥2500
  • 其他:

产品详情

  • 产品描述:
        大鼠降钙素(CT)ELISA试剂盒用于定量测量大鼠血清、血浆或组织匀浆中的降钙素浓度。它在重要特征方面表现良好,包括敏感性和特异性。该测定基于夹心ELISA机制和酶底物显色反应。溶液的颜色与样品中降钙素的含量成正比。并且可以通过微板读取器在450纳米处测量颜色的强度。
        降钙素是一种由甲状腺C细胞分泌的激素,主要用于降低血液中的钙水平。它通过抑制骨骼中破骨细胞的活性和增加尿液中排出的钙量来降低血钙水平。这种激素还阻止钙从肠道吸收,并增加肾脏吸收的钙。当血清中钙浓度升高时,降钙素被诱导表达,并防止高钙血症的发展。降钙素也受到胃泌素等胃肠激素的刺激。它也是甲状腺癌的重要肿瘤标志物。
  • 别名:
    CALCA Calcitonin
  • 缩写:
    CALCA
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1.56 pg/mL-100 pg/mL
  • 灵敏度:
    0.39 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 87-101  
    1:2 Average % 88  
    Range % 80-95  
    1:4 Average % 95  
    Range % 86-106  
    1:8 Average % 90  
    Range % 82-100  
  • 回收率:
    The recovery of rat CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 85-99  
    EDTA plasma (n=4) 94 88-101  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    100 2.746 2.779 2.763 2.582  
    50 2.138 2.175 2.157 1.976  
    25 1.395 1.453 1.424 1.243  
    12.5 0.866 0.854 0.860 0.679  
    6.25 0.552 0.562 0.557 0.376  
    3.12 0.428 0.401 0.415 0.234  
    1.56 0.283 0.298 0.291 0.110  
    0 0.179 0.183 0.181    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

问答及客户评论

 常见问题解答
Q:

我需要一种高灵敏度的降钙素ELISA试剂盒,因为我上次使用的试剂盒灵敏度不够,不能检测血浆中的降钙素水平。你们能否保证可以将降钙素的检测范围限制在2 pg/ml以下?

A:
对于CSB-E05132r试剂盒,目前的灵敏度最低值为0.39 pg/ml,稳定检测范围为1.56 pg/ml-100 pg/ml。如果您的样品浓度低于0.39 pg/ml,那么试剂盒无法检测出来。如果您的样品浓度在0.39 pg/ml-1.56 pg/ml之间,那么试剂盒可以检测出来但是重复性不是很好。

靶点详情

  • 功能:
    Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    Calcitonin family
  • 数据库链接: