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Rat Apoptosis regulator BAX(BAX) ELISA kit

  • 中文名称：
    大鼠细胞凋亡调节因子BAX(BAX)酶联免疫试剂盒
  • 货号：
    CSB-EL002573RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        BAX (Bcl-2-associated X protein)是一种编码细胞凋亡的蛋白质，是Bcl-2家族的一个成员。它通过释放细胞质色素C促进凋亡。BAX是细胞凋亡通路中的关键分子，参与调控线粒体的透性，从而导致细胞凋亡。BAX在正常生理过程中也具有重要作用。它在正常细胞的有丝分裂中起到促进凋亡的作用，使得异常细胞更容易被身体识别和清除。
        j9九游会登录入口首页生物所提供的Rat Apoptosis regulator BAX(BAX) ELISA kit属于ELISA检测试剂盒，采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆、细胞裂解液样本中的BAX，其灵敏度为15.6 pg/mL，检测范围为62.5 pg/mL-4000 pg/mL。
     
  • 别名：
    Bax; Apoptosis regulator BAX
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 85-92  
    1:2 Average % 96  
    Range % 91-105  
    1:4 Average % 90  
    Range % 87-99  
    1:8 Average % 93  
    Range % 82-98  
  • 回收率：
    The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-97  
    EDTA plasma (n=4) 97 93-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.091 1.995 2.043 1.844  
    2000 1.751 1.776 1.764 1.565  
    1000 1.368 1.432 1.400 1.201  
    500 0.985 0.972 0.979 0.780  
    250 0.535 0.544 0.540 0.341  
    125 0.348 0.352 0.350 0.151  
    62.5 0.244 0.237 0.241 0.042  
    0 0.201 0.197 0.199    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    BAX是一种参与调控细胞凋亡的蛋白质，属于Bcl-2家族。最近的研究表明，BAX不仅在细胞凋亡中发挥重要作用，而且在多种疾病的发生中也具有重要作用。例如，BAX在癌症、神经系统疾病、肝脏疾病等的发生中都发挥了重要作用。此外，一些研究还发现BAX在代谢调节、免疫系统调节等方面也具有重要作用。
  • 功能：
    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • 亚细胞定位：
    [Isoform Alpha]: Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm.; [Isoform Beta]: Cytoplasm.; [Isoform Gamma]: Cytoplasm.
  • 蛋白家族：
    Bcl-2 family
  • 组织特异性：
    Expressed in a wide variety of tissues, with highest levels in the testis and ovary.
  • 数据库链接：