Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat Apolipoprotein C-III(APOC3) ELISA kit

Rat Apolipoprotein C-III(APOC3) ELISA kit

  • 中文名称：
    大鼠载脂蛋白C-III(APOC3)酶联免疫试剂盒
  • 货号：
    CSB-EL001933RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat APOC3 ELISA Kit was designed for the quantitative measurement of Rat APOC3 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.8 ng/mL.

  • 别名：
    Apoc3 ELISA Kit; Apolipoprotein C-III ELISA Kit; Apo-CIII ELISA Kit; ApoC-III ELISA Kit; Apolipoprotein C3 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 ng/mL-2000 ng/mL
  • 灵敏度：
    7.8 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat APOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 92    
    Range % 88-96    
    1:200 Average % 95    
    Range % 91-99    
    1:400 Average % 93    
    Range % 90-97    
    1:800 Average % 95    
    Range % 89-99    
  • 回收率：
    The recovery of rat APOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 91 87-93    
    EDTA plasma (n=4) 87 84-90    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    2000 0.093 0.089 0.091      
    1000 0.152 0.144 0.148      
    500 0.227 0.218 0.223      
    250 0.336 0.328 0.332      
    125 0.498 0.488 0.493      
    62.5 0.657 0.640 0.649      
    31.25 0.963 0.893 0.928      
    0 1.673 1.583 1.628      
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein C3 family
  • 组织特异性：
    Synthesized predominantly in liver and to a lesser degree in intestine.
  • 数据库链接：