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Rat Agrin(AGRN) ELISA kit

  • 中文名称：
    大鼠聚集蛋白(AGRN)酶联免疫试剂盒
  • 货号：
    CSB-EL001461RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Agrin (AGRN) ELISA kit is a reliable and accurate tool for measuring Agrin protein levels in samples from Rattus norvegicus (Rat). Agrin, encoded by the AGRN gene, is a protein that plays a crucial role in the development and maintenance of the neuromuscular junction.

    This ELISA kit provides a simple and sensitive assay to quantify Rat Agrin in serum, plasma, and tissue homogenates with a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 19.5 pg/mL. The assay can be completed in just 1-5 hours, making it an efficient and convenient tool for research purposes.

    The Rat Agrin (AGRN) ELISA kit requires a sample volume of only 50-100 µl, minimizing the need for large sample sizes. Detection is performed at a wavelength of 450 nm, with results displayed in a standard curve format.

  • 别名：
    Agrn ELISA kit; AgrinAgrin [Cleaved into: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment ELISA kit; C90); Agrin C-terminal 22 kDa fragment ELISA kit; C22)] ELISA kit
  • 缩写：
    AGRN
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 90-96  
    1:2 Average % 84  
    Range % 80-88  
    1:4 Average % 83  
    Range % 80-86  
    1:8 Average % 89  
    Range % 86-91  
  • 回收率：
    The recovery of rat AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 97-103  
    EDTA plasma (n=4) 96 93-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    5000 2.177 2.198 2.188 2.091  
    2500 1.847 1.863 1.855 1.758  
    1250 1.293 1.273 1.283 1.186  
    625 0.811 0.846 0.829 0.732  
    312.5 0.505 0.496 0.501 0.404  
    156 0.301 0.321 0.311 0.214  
    78 0.188 0.189 0.189 0.092  
    0 0.096 0.098 0.097    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.; Isoform 1, isoform 4, isoform 5 and isoform 6: neuron-specific (z+) isoforms that contain C-terminal insertions of 8-19 AA are potent activators of AChR clustering. Isoform 5, agrin (z+8), containing the 8-AA insert, forms a receptor complex in myotubules containing the neuronal AGRN, the muscle-specific kinase MUSK and LRP4, a member of the LDL receptor family. The splicing factors, NOVA1 and NOVA2, regulate AGRN splicing and production of the 'z' isoforms.; is involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling.; this released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia.
  • 基因功能参考文献：
    1. compared expression of agrin isoforms with the expression of AChE-T and ColQ in the developing spinal cord at the stages before and after the formation of NMJ basal lamina PMID: 23117006
    2. present the first crystal structure of an agrin-LRP4 complex which is essential for neuronal agrin-induced acetylcholine receptor (AChR) clustering PMID: 22302937
    3. agrin modulates neuropathic pain through NR1 phosphorylation in GABA neurons PMID: 21068333
    4. a model wherein the z8 insert is a Ca(2+)-responsive allosteric element that is essential in forming an active conformation in neuronal a PMID: 20566625
    5. Agrin binds BMP2, BMP4 and TGFbeta1 with relatively high affinity. PMID: 20505824
    6. different regions within the agrin protein are responsible for synapse formation at the neuromuscular junction and for process formation in central nervous system neurons PMID: 19940118
    7. The expression of agrin was studied during muscle development in a novel three-dimensional muscle-neuronal coculture system. PMID: 12955494
    8. Agrin deletion mutants show that axonal targeting of agrin depends on multiple domains that function in an additive fashion, including the very N-terminal portions and the C-terminal half of the molecule. PMID: 14697677
    9. Agrn becomes concentrated at neuroeffector and neuromuscular juncions in developing urinary bladder. PMID: 15711988
    10. Agrin may play a pivotal role in synaptogenesis in promoting a rapid switch between electrical coupling and synaptic neurotransmission. PMID: 15883200
    11. Agrin plays a role as a regulator of intercellular communication particularly junctional electric coupling and synapatic remodeling. PMID: 16274639
    12. Results suggest that by positively regulating filopodia in developing neurons, transmembrane agrin may influence the pattern of neurite outgrowth and synapse formation. PMID: 16860570
    13. Agrin, released from neurons, controls the maturation of the excitation-contraction coupling mechanism and due to modulation of RyRs and L-type Ca(2+) channels. A role for neural agrin in skeletal muscle maturation is proposed. PMID: 18003748
    14. Agrin plays an important role in the sprouting phase of reactive synaptogenesis; both its expression and distribution are correlated with extent of successful recovery after TBI. PMID: 18627255
    15. increasing the concentration of an ECM molecule, i.e. agrin, may enhance nicotine effects on pCREB PMID: 18639611
    16. transient post-ischemic hypothermia stabilizes brain vessels and reduces blood brain barrier disruption in part by preventing proteolytic degradation of regulatory basement membrane constituents, SPARC and agrin PMID: 19285050
    17. The results suggest a physiological role for Tm-agrin in the maturation of hippocampal neurons involving positive regulation of dendritic filopodia and promotion of synaptogenesis, but also suggest a role for axonal agrin in synaptogenesis. PMID: 19524020

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  • 亚细胞定位：
    Cell junction, synapse. Cell membrane; Single-pass type II membrane protein.
  • 组织特异性：
    Embryonic nervous system and muscle.
  • 数据库链接：

    KEGG: rno:25592

    STRING: 10116.ENSRNOP00000050623

    UniGene: Rn.2163