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  4. Rat Acylated Ghrelin (AG) ELISA Kit

Rat Acylated Ghrelin (AG) ELISA Kit

  • 中文名称：
    大鼠酰基化饥饿素(AG)酶联免疫试剂盒
  • 货号：
    CSB-E13167r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Acylated Ghrelin (AG) ELISA Kit is a cutting-edge solution for the accurate and efficient detection and quantification of Acylated Ghrelin in rat samples. Acylated Ghrelin is a hormone produced in the stomach that has been implicated in a variety of physiological processes. This ELISA kit is designed to detect and measure Acylated Ghrelin in rat serum, plasma, and tissue homogenates, with a detection range of 6.25 pg/mL-400 pg/mL and a sensitivity of 1.56 pg/mL. This provides precise and accurate results, allowing for the measurement of even the smallest amounts of Acylated Ghrelin in your samples.

    The assay time ranges from 1-5 hours, and requires only a small sample volume of 50-100ul, making our ELISA kit a quick and efficient solution for your research needs. The detection wavelength of 450 nm provides clear and reliable readings.

    It operates using a sandwich assay principle, ensuring accurate and precise measurements of Acylated Ghrelin in your samples. The kit's high sensitivity and specificity make it an essential tool for researchers investigating the role of Acylated Ghrelin in various physiological processes.

  • 缩写：
    AG
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %87-101
    1:2Average %86
    Range %80-92
    1:4Average %93
    Range %90-95
    1:8Average %95
    Range %92-98
  • 回收率：
    The recovery of rat AG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10095-104
    EDTA plasma (n=4)9795-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.690 2.699 2.695 2.502
    2001.885 1.899 1.892 1.699
    1001.162 1.198 1.180 0.987
    500.798 0.760 0.779 0.586
    250.581 0.535 0.558 0.365
    12.50.388 0.376 0.382 0.189
    6.250.301 0.321 0.311 0.118
    00.191 0.195 0.193 　
  • 数据处理：
  • 货期：
    3-5 working days

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