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  4. Rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

Rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

  • 中文名称：
    兔子基质金属蛋白酶抑制因子1(TIMP-1)酶联免疫试剂盒
  • 货号：
    CSB-E06935Rb
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Rabbit TIMP1 ELISA Kit was designed for the quantitative measurement of Rabbit TIMP1 protein in serum, plasma, cell culture supernates , tissue homogenates. It is a Competitive ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 别名：
    TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, cell culture supernates , tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 90  
    Range % 85-97  
    1:200 Average % 102  
    Range % 98-108  
    1:400 Average % 98  
    Range % 94-102  
    1:800 Average % 88  
    Range % 84-93  
  • 回收率：
    The recovery of rabbit TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.086 0.084 0.085    
    500 0.145 0.141 0.143    
    250 0.245 0.238 0.242    
    125 0.392 0.413 0.403    
    62.5 0.725 0.727 0.726    
    31.2 0.963 0.944 0.954    
    15.6 1.154 1.179 1.167    
    0 1.734 1.662 1.698    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
  • 基因功能参考文献：
    1. Hemoperfusion could obviously reduce oxidative stress and the expression levels of MMP-2, MMP-9 and TIMP-1 in rabbits with acute paraquat poisoning. PMID: 22801080
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Protease inhibitor I35 (TIMP) family
  • 数据库链接：