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Rabbit matrix metalloproteinase 3,MMP-3 ELISA kit

  • 中文名称：
    兔子基质金属蛋白酶3(MMP-3)酶联免疫试剂盒
  • 货号：
    CSB-E09269Rb
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Rabbit MMP3 ELISA Kit was designed for the quantitative measurement of Rabbit MMP3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    MMP3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; Transin-1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:5 Average % 84  
    Range % 80-94  
    1:10 Average % 97  
    Range % 91-105  
    1:20 Average % 93  
    Range % 85-97  
    1:40 Average % 94  
    Range % 86-99  
  • 回收率：
    The recovery of rabbit MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-98  
    EDTA plasma (n=4) 89 85-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.794 2.694 2.744 2.593  
    5 2.244 2.144 2.194 2.043  
    2.5 1.424 1.324 1.374 1.223  
    1.25 0.854 0.824 0.839 0.688  
    0.625 0.546 0.536 0.541 0.390  
    0.312 0.361 0.351 0.356 0.205  
    0.156 0.230 0.220 0.225 0.074  
    0 0.152 0.149 0.151 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 基因功能参考文献：
    1. Compromised autophagy may be related to the osteoarthritis progression; JNK and p38 MAPKs up-regulate MMP3 and down-regulate autophagy. PMID: 26873249
    2. the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts. PMID: 23879595
    3. chitosan-pDNA nanoparticles encoding shRNA targeting MMP-3 and -13 had great potential in silencing the dedifferentiation-related genes for regenerating prolonged and endurable cartilage. PMID: 23520014
    4. Ulinastatin effectively inhibited the increased expression of MMP-2, MMP-3, and iNOS in degenerated NP cells induced by IL-1beta in vitro. PMID: 22853554
    5. Exposure to long periods of light irrespective of its characteristics leads to the increased expression of some matrix metalloprpteases. PMID: 20238014
    6. Tongxinluo can inhibit the expression of MMP-3 and 9 and increase the expression of PPARgamma in atherosclerotic rabbits. PMID: 19778818

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：