Rabbit Tartrate-resistant acid phosphatase 5b (TRAP5b) ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Rabbit TRAP5b ELISA Kit was designed for the quantitative measurement of Rabbit TRAP5b protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 μU/mL-5000 μU/mL and the sensitivity is 19.5 μU/mL.
别名：

ACP5 ELISA Kit; Tartrate-resistant acid phosphatase type 5 ELISA Kit; TR-AP ELISA Kit; EC 3.1.3.2 ELISA Kit; Tartrate-resistant acid ATPase ELISA Kit; TrATPase ELISA Kit; Type 5 acid phosphatase ELISA Kit
缩写：

TRAP5b
Uniprot No.：

O97860
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

78 μU/mL-5000 μU/mL
灵敏度：

19.5 μU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TRAP5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-101
1:2	Average %	88
	Range %	81-94
1:4	Average %	97
	Range %	87-101
1:8	Average %	91
	Range %	84-95

回收率：

The recovery of rabbit TRAP5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-96
EDTA plasma (n=4)	103	93-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.