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Rabbit Interleukin 1,IL-1 ELISA Kit

  • 中文名称：
    兔子白介素1(IL-1)酶联免疫试剂盒
  • 货号：
    CSB-E06896Rb
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    The rabbit IL-1 ELISA kit is suitable for the quantitative determination of rabbit IL-1 in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.

    IL-1, also called IL-1α or IL1A, is a ubiquitous and pivotal pro-inflammatory cytokine. IL-1/IL-1R1 signaling induces pro-inflammatory signaling cascades including NF-κB, JNK, p38, AP-1, and MAPK pathways, resulting in the expression of myriad mediators, which orchestrate the development of an immune response. It exerts both beneficial, promoting innate immunity against invading microorganisms, and harmful roles in a variety of autoimmune and autoinflammatory diseases including cancer. In cancers, IL-1 affects the tumor microenvironment and promotes the initiation and progression of tumor cells.

  • 别名：
    IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
  • 缩写：
    IL-1
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %84-96
    1:2Average %92
    Range %85-100
    1:4Average %95
    Range %89-101
    1:8Average %93
    Range %87-99
  • 回收率：
    The recovery of rabbit IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9688-99
    EDTA plasma (n=4)9284-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.772 2.641 2.707 2.547
    5002.216 2.068 2.142 1.982
    2501.286 1.186 1.236 1.076
    1250.652 0.634 0.643 0.483
    62.50.370 0.355 0.363 0.203
    31.20.282 0.264 0.273 0.113
    15.60.199 0.212 0.206 0.046
    00.164 0.156 0.160 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 基因功能参考文献：
    1. We propose that induction of collagenase-1 by IL-1alpha in both WF and NF depends on a unique combination of cell type-specific signaling pathways. PMID: 17455222
  • 亚细胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：