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  4. Pig terminal complement complex C5b-9 (TCC C5b-9)ELISA Kit

Pig terminal complement complex C5b-9 (TCC C5b-9)ELISA Kit

  • 中文名称：
    猪末端补体复合物C5b-9(TCC C5b-9)酶联免疫试剂盒
  • 货号：
    CSB-E15837p
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig TCC C5b-9 ELISA Kit was designed for the quantitative measurement of Pig TCC C5b-9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 ng/mL-1200 ng/mL and the sensitivity is 4.69 ng/mL.

  • 缩写：
    TCC C5b-9
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    18.75 ng/mL-1200 ng/mL
  • 灵敏度：
    4.69 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %99
    Range %85-105
    1:2Average %95
    Range %85-113
    1:4Average %95
    Range %82-102
    1:8Average %97
    Range %85-110
  • 回收率：
    The recovery of pig TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9688-109
    EDTA plasma (n=4)9785-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    12002.217 2.319 2.268 2.130
    6001.790 1.767 1.779 1.641
    3001.123 1.132 1.128 0.990
    1500.675 0.651 0.663 0.525
    750.397 0.403 0.400 0.262
    37.50.285 0.298 0.292 0.154
    18.750.231 0.216 0.224 0.086
    00.141 0.134 0.138 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

At what wavelength do i read the results?

A:
Thanks for your inquiry.
CSB-E15837p
The detection wavelength must be 450nm.
The absorbance value will be very good at this wavelength. Considering there may be scratches at the bottom of the vial when you add samples, we suggest you use dual wavelength( detection wavelength and correction wavelength).
Pls let me know if you have any further questions. Thank you.