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  4. Pig neutrophil gelatinase-associated lipocalin(NGAL)ELISA Kit

Pig neutrophil gelatinase-associated lipocalin(NGAL)ELISA Kit

  • 中文名称：
    猪中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫试剂盒
  • 货号：
    CSB-E16507p
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig NGAL ELISA Kit was designed for the quantitative measurement of Pig NGAL protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

  • 缩写：
    NGAL
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:200 Average % 105
    Range % 101-108
    1:400 Average % 96
    Range % 92-98
    1:800 Average % 96
    Range % 90-99
    1:1600 Average % 89
    Range % 84-93
  • 回收率：
    The recovery of pig NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 87-94
    EDTA plasma (n=4) 99 99-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average
    500 0.132 0.135 0.134
    250 0.254 0.265 0.260
    125 0.612 0.563 0.588
    62.5 1.054 1.068 1.061
    31.2 1.538 1.652 1.595
    15.6 2.079 2.122 2.101
    7.8 2.435 2.454 2.445
    0 2.841 2.878 2.860
  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

I would like to be sure it is good both for urine and serum. Could you confirm?

A:
Thanks for your inquiry. We tested serum and plasma samples under 1:500 dilution before and the test value is 30--125pg/ml with this kit CSB-E16507p.
The final concentration is 15--62.5ng/ml. We suggest you order 24T sample kit and do a pretest first if you want to test urine samples. Pls let me know if you have any further questions. Thank you.