Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Pig monocyte chemotactic protein 1,MCP1 ELISA Kit

Pig monocyte chemotactic protein 1,MCP1 ELISA Kit

  • 中文名称：
    猪单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)酶联免疫试剂盒
  • 货号：
    CSB-E13178p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig CCL2 ELISA Kit was designed for the quantitative measurement of Pig CCL2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig MCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %87-96
    1:2Average %98
    Range %93-102
    1:4Average %85
    Range %81-89
    1:8Average %96
    Range %93-99
  • 回收率：
    The recovery of pig MCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9188-96
    EDTA plasma (n=4)8580-90
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.078 2.069 2.074 1.880
    10001.610 1.687 1.649 1.455
    5001.099 1.059 1.079 0.885
    2500.615 0.638 0.627 0.433
    1250.427 0.438 0.433 0.239
    62.50.342 0.349 0.346 0.152
    31.250.276 0.268 0.272 0.078
    00.189 0.198 0.194
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
  • 基因功能参考文献：
    1. these results provide novel insights into CCL2-mediated signaling mechanisms in the porcine endometrium at the maternal-fetal interface during early pregnancy PMID: 29278679
    2. CXCL10 protein was detected in serum from pigs infected with a high virulence African swine fever virus isolate. Levels of CCL2 mRNA were increased in pigs infected with the high virulence isolate compared to a low virulence isolate. PMID: 24083897
    3. Porcine CCL2 binding on Tuk4+SLA-II- but not on Tuk4-SLA-II+ cells was detected by using a CCL2-green fluorescence protein fusion protein. PMID: 20670605
    4. The protective properties of flavonoids, such as EGCG, against endothelial inflammation may be regulated in part though induction of HO-1 and subsequent activator protein-1 signaling. PMID: 20580034
    5. MCP-1 contributes to functional and structural impairment in the kidney in renal artery stenosis, mainly in the tubulointerstitial compartment PMID: 19730125
    6. may contribute to xenograft rejection by attracting monocytes into the islets PMID: 14962280
    7. Contrast media did not affect bone marrow cell viability/MCP-1 secretion. PMID: 15274157
    8. Porcine vessel wall injury via balloon arthroplasty upregulates expression of CCL2 by coronary artery transmural and perivascular cells in a sequential pattern. PMID: 16926016
    9. in the aortic arch expression of monocyte chemoattractant protein 1 (MCP-1) was upregulated, compared with the thoracic aorta PMID: 17588585
    10. Variable gene expression (eg, matrix metalloproteinase-9, CCL2 and Lp-PLA(2) mRNAs), both in regard to the arterial bed and duration of disease, was associated with variable plaque development and progression. PMID: 18276914
    11. myocardial infarction induced proinflammatory gene and protein expression in peripheral blood mononuclear cells of tissue factor cyclo-oxygenase-2, monocyte chemoattractant protein-1 and CRP PMID: 19036073

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 数据库链接：

    KEGG: ssc:397422

    STRING: 9823.ENSSSCP00000018779

    UniGene: Ssc.657