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  4. Pig major acute phase protein (MAP) ELISA Kit

Pig major acute phase protein (MAP) ELISA Kit

  • 中文名称：
    猪主要急性期蛋白( MAP)酶联免疫试剂盒
  • 货号：
    CSB-E13425p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig ITIH4 ELISA Kit was designed for the quantitative measurement of Pig ITIH4 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 37.5 ng/mL-2400 ng/mL and the sensitivity is 9.38 ng/mL.

  • 别名：
    ITIH4 ELISA Kit; IHRP ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; IHRP ELISA Kit; Major acute phase protein ELISA Kit; MAP ELISA Kit
  • 缩写：
    ITIH4
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    37.5 ng/mL-2400 ng/mL
  • 灵敏度：
    9.38 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig MAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 97  
    Range % 92-104  
    1:400 Average % 95  
    Range % 91-99  
    1:800 Average % 93  
    Range % 87-99  
    1:1600 Average % 86  
    Range % 82-92  
  • 回收率：
    The recovery of pig MAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-101  
    EDTA plasma (n=4) 97 90-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2400 0.138 0.141 0.140    
    1200 0.457 0.460 0.459    
    600 0.903 0.898 0.901    
    300 1.134 1.121 1.128    
    150 1.518 1.483 1.501    
    75 1.689 1.706 1.698    
    37.5 2.032 2.049 2.041    
    0 2.187 2.114 2.151    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.
  • 基因功能参考文献：
    1. Promoter of ITIH4 major acute phase protein has been isolated, cloned, and proven functional by response to an inflammatory stimulus (interleukin-6) in human Hep3B cells. (inter-alpha-trypsin heavy chain 4; ITIH4) PMID: 16275952
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ITIH family
  • 组织特异性：
    Liver specific.
  • 数据库链接：