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  4. Pig glial fibrillary acidic protein,GFAP ELISA Kit

Pig glial fibrillary acidic protein,GFAP ELISA Kit

  • 中文名称：
    猪神经胶质纤维酸性蛋白(GFAP)酶联免疫试剂盒
  • 货号：
    CSB-E14950p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig GFAP ELISA Kit was designed for the quantitative measurement of Pig GFAP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.1 ng/mL-20 ng/mL and the sensitivity is 0.05 ng/mL.

  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.1 ng/mL-20 ng/mL
  • 灵敏度：
    0.05 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 84-97  
    1:2 Average % 96  
    Range % 92-99  
    1:4 Average % 92  
    Range % 89-96  
    1:8 Average % 96  
    Range % 90-102  
  • 回收率：
    The recovery of pig GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-105  
    EDTA plasma (n=4) 96 92-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    20 0.187 0.195 0.191    
    5 0.338 0.346 0.342    
    1.6 0.559 0.551 0.555    
    0.4 0.842 0.868 0.855    
    0.1 1.334 1.337 1.336    
    0 1.989 2.005 1.997    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I have the question about the antibody of this kit since it is being compared to another Elisa kit.
1) Is this a GFAP antibody that recognizes the full length or just the C terminus?
2) The source of the GFAP antibody ? Is it from cow spinal cord?

A:
Thanks for your inquiry.
CSB-E14950p
This kit is designed against full length protein.
The plate is coated with goat-anti-rabbit antibody. The specific antibody used in the kit is rabbit polyclonal antibody.Pls let me know if you have any further questions. Thank you.