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  4. Pig collagen type I (COLI) ELISA kit

Pig collagen type I (COLI) ELISA kit

  • 中文名称：
    猪I型胶原(COLI)酶联免疫试剂盒
  • 货号：
    CSB-EQ027853PI
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig COLI ELISA Kit was designed for the quantitative measurement of Pig COLI protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

  • 别名：
    collagen type 1 (COL1),_x000D_ collagen type I (COLI)
  • 缩写：
    COLI
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig COLⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 88    
    Range % 86-91    
    1:2 Average % 89    
    Range % 81-95    
    1:4 Average % 98    
    Range % 93-104    
    1:8 Average % 102    
    Range % 98-106    
  • 回收率：
    The recovery of pig COLⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 104 94-109    
    EDTA plasma (n=4) 92 88-98    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    5000 2.621 2.731 2.676 2.501    
    2500 2.155 2.177 2.166 1.991    
    1250 1.503 1.455 1.479 1.304    
    625 0.983 0.930 0.957 0.782    
    312 0.672 0.696 0.684 0.509    
    156 0.451 0.444 0.448 0.273    
    78 0.315 0.329 0.322 0.147    
    0 0.174 0.176 0.175 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I would like to confirm that the COL1 ELISA Kit, is specific for pig collagen type 1

A:
Thanks for your inquiry!The kit csb-eq027853pi specifically detects pig collagen type I.
■ 客户评论
  • Tested application: ELISA
    Test sample: Protein (recombinant/purified
    Species: pig
    Review: The repeatabilityis good but this method doesn't detect our pig protein.