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  4. Pig basic fibroblast growth factor,bFGF ELISA Kit

Pig basic fibroblast growth factor,bFGF ELISA Kit

  • 中文名称：
    猪碱性成纤维细胞生长因子(bFGF)酶联免疫试剂盒
  • 货号：
    CSB-E12841p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig FGF2 ELISA Kit was designed for the quantitative measurement of Pig FGF2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 pg/mL-300 pg/mL and the sensitivity is 1.2 pg/mL.

  • 别名：
    FGF2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit; Prostatic growth factor ELISA Kit; Fragments ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    4.7 pg/mL-300 pg/mL
  • 灵敏度：
    1.2 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 80-100  
    1:2 Average % 97  
    Range % 91-105  
    1:4 Average % 94  
    Range % 90-110  
    1:8 Average % 95  
    Range % 82-105  
  • 回收率：
    The recovery of pig bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 85-104  
    EDTA plasma (n=4) 98 92-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    300 2.175 2.157 2.166 1.966  
    150 1.582 1.612 1.597 1.397  
    75 1.202 1.134 1.168 0.968  
    37.5 0.628 0.603 0.616 0.416  
    18.75 0.377 0.362 0.370 0.170  
    9.4 0.296 0.301 0.299 0.099  
    4.7 0.265 0.256 0.261 0.061  
    0 0.196 0.204 0.200 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
  • 亚细胞定位：
    Secreted. Nucleus.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 数据库链接：