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Pig Soluble protein-100B,S-100B ELISA Kit

  • 中文名称:
    猪S100B蛋白(S-100B)酶联免疫试剂盒
  • 货号:
    CSB-E14063p
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:

    This Pig S-100B ELISA Kit was designed for the quantitative measurement of Pig S-100B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL .

  • 缩写:
    S-100B
  • Uniprot No.:
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    78 pg/mL-5000 pg/mL
  • 灵敏度:
    19.5 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %95
    Range %91-100
    1:2Average %93
    Range %85-101
    1:4Average %86
    Range %80-98
    1:8Average %94
    Range %92-105
  • 回收率:
    The recovery of pig S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9583-102
    EDTA plasma (n=4)9592-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.025 2.067 2.046 1.887
    25001.574 1.524 1.549 1.390
    12501.183 1.169 1.176 1.017
    6250.821 0.812 0.817 0.658
    3120.567 0.534 0.551 0.392
    1560.354 0.373 0.364 0.205
    780.250 0.262 0.256 0.097
    00.164 0.153 0.159  
  • 数据处理:
  • 货期:
    3-5 working days

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