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  4. Pig Renin ELISA Kit

Pig Renin ELISA Kit

  • 中文名称：
    猪肾素(Renin)酶联免疫试剂盒
  • 货号：
    CSB-E17914p
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig REN ELISA Kit was designed for the quantitative measurement of Pig REN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    FLJ10761, HNFJ2, angiotensin-forming enzyme|angiotensinogenase|renin precursor, renal
  • 缩写：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 85-103    
    1:2 Average % 92    
    Range % 85-102    
    1:4 Average % 93    
    Range % 83-99    
    1:8 Average % 95    
    Range % 90-110    
  • 回收率：
    The recovery of pig renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 82-102    
    EDTA plasma (n=4) 94 86-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    4000 1.973 1.997 1.985 1.867    
    2000 1.635 1.643 1.639 1.521    
    1000 1.208 1.197 1.203 1.085    
    500 0.805 0.796 0.801 0.683    
    250 0.549 0.544 0.547 0.429    
    125 0.361 0.356 0.359 0.241    
    62.5 0.255 0.259 0.257 0.139    
    0 0.116 0.119 0.118 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I have a customer who interested in both Human renin, REN ELISA Kit CSB-E08701h & Pig Renin ELISA Kit CSB-E17914p
They would like to know if they can use whole blood in these two kits?

A:
Thanks for your inquiry!
Renin, angiotensin constitute renin-angiotensin system (RAS) or renin, angiotensin, aldosterone constitute renin-angiotensin-aldosterone system (RAAS) together to participate in body fluid balance.
Both kits are suitable to serum and plasma samples.They are for quantitative measurement of renin. To test whole blood is not advised.
Pls let me know if you have any further questions. Thank you.