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Pig Interleukin 6,IL-6 ELISA Kit

  • 中文名称：
    猪白介素6(IL-6)酶联免疫试剂盒
  • 货号：
    CSB-E06786p
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        白细胞介素6（Interleukin-6，IL-6）是一种由多种细胞产生的细胞因子，包括巨噬细胞、T细胞和B细胞。IL-6参与了人体的多种生理和病理过程，如免疫反应、急性期反应和炎症反应等。IL-6的水平通常在感染、炎症和组织损伤等情况下升高。IL-6也与一些疾病的发生和发展相关，如类风湿关节炎、炎症性肠病和癌症等。
        j9九游会登录入口首页生物所提供的Pig Interleukin 6,IL-6 ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测猪血清、血浆、组织匀浆样本中的IL6，其灵敏度为0.31 pg/mL，检测范围为1.25 pg/mL-80 pg/mL。
     
  • 别名：
    IL6 ELISA Kit; Interleukin-6 ELISA Kit; IL-6 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.25 pg/mL-80 pg/mL
  • 灵敏度：
    0.31 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 86  
    Range % 80-90  
    1:2 Average % 95  
    Range % 91-100  
    1:4 Average % 97  
    Range % 95-103  
    1:8 Average % 90  
    Range % 85-95  
  • 回收率：
    The recovery of pig IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-95  
    EDTA plasma (n=4) 99 95-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    80 2.212 2.101 2.157 2.066  
    40 1.725 1.704 1.715 1.624  
    20 1.223 1.194 1.209 1.118  
    10 0.871 0.809 0.840 0.749  
    5 0.519 0.504 0.512 0.421  
    2.5 0.332 0.315 0.324 0.233  
    1.25 0.192 0.182 0.187 0.096  
    0 0.093 0.089 0.091 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 2 个评价

样品类型：细胞培养上清液

样品信息：其他

稀释比：没有稀释

产品评价： 结果很满意，也是对比了别家的试剂盒，最后发现还是j9九游会登录入口首页的好用，我用的是细胞培养基上清，别家的试剂盒有干扰因素，j9九游会登录入口首页的没有，试了好几家，也就这个牌子的好用。

By 高老师

样品类型：血清

样品信息：其他 猪

稀释比：没有稀释

产品评价： 出于一直以来对j9九游会登录入口首页产品的信任，这一次测定猪血清还是使用了j9九游会登录入口首页的试剂盒，IL-6检测不明显，加大样本量希望再看情况如何。

By 杨老师

问答及客户评论

■ 常见问题解答
Q:

我有一份已经在1500 g下离心15分钟的猪血清样本。但是，这个试剂盒CSB-E06786p的说明书建议以1000 g离心15分钟。我想知道这是否会对结果产生影响。

A:
本手册建议以1000g15 min离心。上述1500 g、15 min的离心机在理论上影响不大。建议您主要检查是否发生了溶血，或者离心机是否在低温下运行，因为离心过程中的温度会随着速度和时间的增加而升高。

靶点详情

  • 最新研究进展：
    IL-6是一种重要的细胞因子，参与多种生物学过程的调节，如炎症反应、免疫应答和细胞增殖等。最新研究发现，IL-6与COVID-19感染的严重程度密切相关，高水平的IL-6水平与COVID-19患者的死亡率升高有关。同时，IL-6受体抑制剂托珠单抗（Tocilizumab）可用于治疗COVID-19患者的炎症反应和肺损伤。此外，研究还表明，IL-6在许多癌症中发挥作用，可以作为一种治疗靶点。
  • 功能：
    Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells.; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.
  • 基因功能参考文献：
    1. IL-6 was low or undetectable in Boar seminal plasma. PMID: 22482797
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 数据库链接：

    KEGG: ssc:399500

    STRING: 9823.ENSSSCP00000024026

    UniGene: Ssc.62