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Pig Interleukin 1β,IL-1β ELISA Kit

  • 中文名称：
    猪白介素1β (IL-1β)酶联免疫试剂盒
  • 货号：
    CSB-E06782p
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig IL1B ELISA Kit was designed for the quantitative measurement of Pig IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.9 pg/mL-250 pg/mL and the sensitivity is 0.975 pg/mL .

  • 别名：
    IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    3.9 pg/mL-250 pg/mL
  • 灵敏度：
    0.975 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-100
    1:2Average %96
    Range %91-103
    1:4Average %95
    Range %90-110
    1:8Average %95
    Range %85-105
  • 回收率：
    The recovery of pig IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9583-104
    EDTA plasma (n=4)9890-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2502.675 2.815 2.745 2.597
    1252.154 2.147 2.151 2.003
    62.51.312 1.335 1.324 1.176
    31.250.788 0.764 0.776 0.628
    15.60.377 0.356 0.367 0.219
    7.80.264 0.269 0.267 0.119
    3.90.190 0.188 0.189 0.041
    00.153 0.143 0.148 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型：细胞培养上清液

样品信息：其他

稀释比：没有稀释

产品评价： 结果很满意，也是对比了别家的试剂盒，最后发现还是j9九游会登录入口首页的好用，我用的是细胞培养基上清，别家的试剂盒有干扰因素，j9九游会登录入口首页的没有，试了好几家，也就这个牌子的好用。

By 高老师

相关问答

■ 常见问题解答
Q:

Hello, please advise if the Pig IL-1b ELISA kit (#CSB-E06782p) recognizes only mature IL-1b or both mature and pro-IL-1b protein. Thank you.

A:
Thanks for your inquiry. This kit detects total IL-1b, including mature form and pro form.

靶点详情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 基因功能参考文献：
    1. Analysis of host-protein interactions for African swine fever virus L83L identified IL-1beta as its host ligand. A recombinant African swine fever virus lacking the L83L was developed from the highly virulent field isolate Georgia2007 and was used to show that L83L is a non-essential gene. PMID: 29605728
    2. This study showed that the presence of embryos in oviducts on days 2-3 after mating may influence the oviductal expression of the members of the IL-1beta system, determining the action of IL-1beta, which may be considered to be the earliest sign of pregnancy in pigs. PMID: 28532837
    3. This study showed that classical swine fever virus and p7 protein induced IL-1beta secretion and that p7 protein was a short-lived protein degraded by the proteasome. PMID: 25146005
    4. local expression of IL-1beta and IL-8 in non-bacterial thrombotic endocarditis, Staphylococcus aureus infective endocarditis, animals with S. aureus sepsis without endocarditis and controls PMID: 23879680
    5. IL1B regulates expression of IL1R1 and IL1RAP and stimulates expression of PTGS1 and PTGS2 that are considered to be the most rate-limiting enzymes for endometrial synthesis of prostaglandins during the peri-implantation period of pregnancy in pigs. PMID: 22572995
    6. the results presented here strongly suggest IL-1beta as a key molecule guiding tissue remodelling events after myocardial infarction. PMID: 20678474

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  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：