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Pig Glucagon(GC) ELISA Kit

  • 中文名称：
    猪胰高血糖素(GC)酶联免疫试剂盒
  • 货号：
    CSB-E04854p
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig GCG ELISA Kit was designed for the quantitative measurement of Pig GCG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 125 pg/mL-2000 pg/mL and the sensitivity is 62.5 pg/mL .

  • 别名：
    GCGGlucagon [Cleaved into: Glicentin; Glicentin-related polypeptide ELISA Kit; GRPP); Oxyntomodulin ELISA Kit; OXM ELISA Kit; OXY); Glucagon; Glucagon-like peptide 1 ELISA Kit; GLP-1); Glucagon-like peptide 1(7-37 ELISA Kit; GLP-1(7-37)); Glucagon-like peptide 1(7-36 ELISA Kit; GLP-1(7-36)); Glucagon-like peptide 2 ELISA Kit; GLP-2)] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 pg/mL-2000 pg/mL
  • 灵敏度：
    62.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %85-96
    1:2Average %95
    Range %92-102
    1:4Average %84
    Range %80-88
    1:8Average %90
    Range %85-96
  • 回收率：
    The recovery of pig GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-97
    EDTA plasma (n=4)9690-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2Average
    20000.120 0.111 0.116
    10000.315 0.340 0.328
    5000.601 0.611 0.606
    2501.018 0.945 0.982
    1252.036 1.923 1.980
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.; Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6. Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis.; Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.; Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.; May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
  • 基因功能参考文献：
    1. Data suggest that casein, safflower oil, sucrose, and sweetening agent rebaudioside A stimulate GLP1 release/secretion from enteroendocrine cells. (GLP1 = glucagon-like peptide 1, a peptide fragment derived from proglucagon) PMID: 27142747
    2. The patterns of colocalization of the K cell marker, glucagon-like insulinotropic peptide, and L cell markers, glucagon like peptide-1 and peptide YY, in enteroendocrine cells of the small intestine and colon of mouse and pig, were investigated. PMID: 25378285
  • 亚细胞定位：
    Secreted.; [Glucagon-like peptide 1]: Secreted.
  • 蛋白家族：
    Glucagon family
  • 组织特异性：
    Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.
  • 数据库链接：