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  4. Pig Follicle-stimulating hormone,FSH ELISA Kit

Pig Follicle-stimulating hormone,FSH ELISA Kit

  • 中文名称：
    猪促卵泡素(FSH)酶联免疫试剂盒
  • 货号：
    CSB-E06791p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig FSH ELISA Kit was designed for the quantitative measurement of Pig FSH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 20 mIU/mL-700 mIU/mL and the sensitivity is 10 mIU/mL.

  • 别名：
    FSHBFollitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
  • 缩写：
    FSH
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    20 mIU/mL-700 mIU/mL
  • 灵敏度：
    10 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-94  
    1:2 Average % 94  
    Range % 90-98  
    1:4 Average % 92  
    Range % 87-97  
    1:8 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of pig FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-99  
    EDTA plasma (n=4) 97 91-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    700 1.389 1.416 1.403 1.367  
    250 0.578 0.615 0.597 0.561  
    100 0.317 0.302 0.310 0.274  
    50 0.163 0.154 0.159 0.123  
    20 0.095 0.101 0.098 0.062  
    0 0.037 0.034 0.036 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
  • 基因功能参考文献：
    1. As in vitro experiments in the present study suggested that LHX2 regulated the expression of Fshbeta, it is possible that LHX2 controls FSH synthesis at the transcriptional level. PMID: 22134063
    2. Results showed that the effects of FSHb, ESR, and PRLR genes were significant in the Tibet pig population, and the effective genotypes of the three genes for reproductive traits were BB, BB, and AA, respectively. PMID: 20466637
    3. LHX3 LIM homeodomain transcription factor is involved in activation of the FSH beta-subunit gene in the pituitary gonadotrope cell. PMID: 15271874
    4. Prop-1 participates in the regulation of FSHbeta gene PMID: 15474519
    5. A allele of FSHbeta-1 locus in all the population and FSHbeta-2 locus in pigs of the first parity had the positive effective on TNB, NBA, and WB. PMID: 18065386

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 数据库链接：