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  4. Pig Epithelial growth factor,EGF ELISA Kit

Pig Epithelial growth factor,EGF ELISA Kit

  • 中文名称：
    猪表皮生长因子(EGF)酶联免疫试剂盒
  • 货号：
    CSB-E06788p
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig EGF ELISA Kit was designed for the quantitative measurement of Pig EGF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 250 pg/mL-10000 pg/mL and the sensitivity is 125 pg/mL.

  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    250 pg/mL-10000 pg/mL
  • 灵敏度：
    125 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 97-105  
    1:2 Average % 93  
    Range % 90-96  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 87  
    Range % 83-90  
  • 回收率：
    The recovery of pig EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 86 83-89  
    EDTA plasma (n=4) 101 99-104  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    10000 2.647 2.687 2.667    
    5000 1.839 1.899 1.869    
    2500 1.057 1.067 1.062    
    1250 0.599 0.584 0.592    
    500 0.241 0.231 0.236    
    250 0.112 0.114 0.113    
  • 数据处理：
  • 货期：
    3-5 working days

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